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AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorJacqueline E. Dawson
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This invention provides a purified immortalized human endothelial cell infected with Ehrlichia chaffeensis or Ehrlichia canis. Also provided is a method of simultaneously screening a sample from a human subject for the presence of E. chaffeensis and Rickettsia rickettsii comprising contacting the sample with immortalized human endothelial cells under conditions which allow infection of the cells and detecting the presence of infection, the presence of infection indicating the presence of E. chaffeensis and/or R. rickettsii. The invention also provides a method of screening a sample from a human subject for the presence of E. chaffeensis comprising contacting the sample with endothelial cells under conditions which allow infection of the cells by E. chaffeensis and detecting the presence of infection by E. chaffeensis, the presence of infection by E. chaffeensis indicating the presence of E. chaffeensis in the sample. Finally, the invention provides a method of culturing E. chaffeensis or E. canis comprising contacting E. chaffeensis or E. canis with immortalized human endothelial cells under conditions which allow the propagation of E. chaffeensis or E. canis.
A method of simultaneously screening a sample from a human subject for a presence of Ehrlichia chaffeensis and Rickettsia rickettsii comprising contacting the sample with immortalized human microvascular endothelial cells under conditions which allow infection of the cells and detecting a presence of infection, the presence of infection indicating the presence of Ehrlichia chaffeensis or Rickettsia rickettsii or both.
A method of screening a sample from a human subject for a presence of Ehrlichia chaffeensis comprising contacting the sample with human microvascular endothelial cells under conditions which allow infection of the cells by Ehrlichia chaffeensis and detecting a presence of infection by Ehrlichia chaffeensis, the presence of infection by Ehrlichia chaffeensis indicating the presence of Ehrlichia chaffeensis in the sample.
A method of culturing Ehrlichia chaffeensis or Ehrlichia canis comprising contacting Ehrlichia chaffeensis or Ehrlichia canis with immortalized human microvascular endothelial cells under conditions which allow propagation of Ehrlichia chaffeensis or Ehrlichia canis.
Dependencies are inferred from the claim text.