Method for evaluating drug metabolism and reagent compositions therefor
AssigneePfizer Inc.
InventorsKaoru Shimada, Mayumi Mizutani, Fumiharu Naganeo
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Abstract
This invention relates to a method for evaluating the susceptibility of pharmaceutical drugs to metabolism by a specific cytochrome P-450 isozyme, which comprises contacting the sample compound with a reagent composition prepared by adding the specific cytochrome P-450 isozyme to liver microsomes lacking said specific cytochrome P-450 isozyme in a carrier material. More particularly, this invention relates to a useful in vitro quantitative assay for drug metabolism by P-450 isozymes such as CYP2D6, CYP2C19, and CYP2A6. This invention also relates to a reagent composition which is useful for said in vitro assay.
Claims13
A method for evaluating the susceptibility of a sample compound to metabolism by a specific cytochrome P-450 isozyme, which comprises contacting the sample compound with a reagent composition prepared by adding said specific cytochrome P-450 isozyme to a liver microsome lacking said specific cytochrome P-450 isozyme in a carrier material.
A reagent composition for use in evaluating drug metabolism by a specific cytochrome P-450 isozyme, which comprises a liver microsome lacking said specific P-450, said specific P-450 isozyme and a carrier material.
Dependencies are inferred from the claim text.