Immortalized human keratinocyte cell line
AssigneeWisconsin Alumni Research Foundation
InventorsB. Lynn Allen-Hoffmann, Sandra J. Schlosser, Michael A. Pickart
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Abstract
A spontaneously immortalized human keratinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. The method comprises the steps of obtaining a human stratified squamous epithelial cell culture, wherein the culture comprises human malignant squamous epithelial cells and spontaneously immortalized human keratinocytes, wherein the culture forms a reconstituted epidermis. One then treats the epidermis with a test tumor cell modulation agent and evaluates the growth of the malignant cells within the epidermis.
Claims7
An immortalized human keratinocyte cell line, wherein the cell line is ATCC CRL-12191.
A method of evaluating test compounds for human toxicity comprising the steps of
- a) exposing the test compound to cell line ATCC CRL-12191, and
- b) evaluating the effect of the compound on the cell line.
Dependencies are inferred from the claim text.