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US 5,985,617 AGranted
Microorganisms and methods for overproduction of DAHP by cloned PPS gene
OwnerJames C. Liaoinventor
Life of this patent
Estimating the term…
Abstract
Genetic elements comprising expression vectors and a gene coding for phosphoenol pyruvate synthase is utilized to enhance diversion of carbon resources into the common aromatic pathway and pathways branching therefrom. The overexpression of phosphoenol pyruvate synthase increases DAHP production to near theoretical yields.
Claims29
A method of producing 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) comprising the steps of:
- (a) placing a microorganism with genetic elements comprising pps and tkt genes wherein said genes are expressed by said microorganism in a media comprising glucose solution;
- (b) overexpressing the pps and tkt genes of said microorganisms in the glucose media;
- and
- (c) recovering DAHP from said media.
1 more element
A method of producing tryptophan comprising the steps of:
- (a) placing a microorganism with genetic elements comprising pps, tkt, and serA genes wherein said genes are expressed by said microorganism in a media comprising glucose solution;
- (b) overexpressing the pps, tkt, and serA genes of said microorganisms in the glucose media;
- and
- (c) recovering tryptophan from said media.
1 more element
A method of producing phenylalanine comprising the steps of:
- (a) placing a microorganism with genetic elements comprising pps, tkt, and pheA.sup.fbr genes wherein said genes are expressed by said microorganism in a media comprising glucose solution;
- (b) overexpressing the pps, tkt, and pheA.sup.fbr genes in said microorganisms in said glucose media;
- and
- (c) recovering phenylalanine from said glucose media.
1 more element
A method of producing quinic acid comprising the steps of:
- (a) placing a microorganism with genetic elements comprising pps, tkt, and qad genes wherein said genes are expressed by said microorganism in a media comprising glucose solution;
- (b) overexpressing the pps, tkt, and qad genes in said microorganisms in the glucose media;
- and
- (c) recovering quinic acid from said media.
1 more element
Dependencies are inferred from the claim text.