Method for the determination of glycosylated proteins
AssigneeKyoto Daiichi Kagaku Co., Ltd.
InventorsSatoshi Yonehara, Tsuguki Komori
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Abstract
A method for measuring the amount of glycosylated protein comprising the steps of decomposing glycosylated proteins with protease, causing a redox reaction between the decomposed products and an Amadori compound oxidoreductase, and determining the redox reaction so as to measure the amount of glycosylated proteins, wherein the decomposition with the protease is carried out in the presence of at least one substrate selected from the group consisting of metalloporphyrin, cytochrome and diaphorase.
Claims10
A method for measuring the amount of glycosylated protein comprising the steps of decomposing glycosylated proteins in a sample with protease, causing a redox reaction between the decomposed products and an Amadori compound oxidoreductase, and determining the redox reaction so as to measure the amount of glycosylated proteins, wherein the decomposition with the protease is carried out in the presence of at least one substance selected from the group consisting of metalloporphyrin, cytochrome and diaphorase.
Dependencies are inferred from the claim text.