Substantially pure histidine-linked protein polymer conjugates
AssigneeEnzon, Inc.
InventorsSeoju Lee, Charles McNemar
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Abstract
Substantially pure histidine-linked protein-polymer conjugates and processes for their preparation are disclosed. The processes include contacting a protein with an activated polymer under conditions sufficient to facilitate covalent attachment of at least a portion of the polymer strands on histidine residues of the protein and thereafter substantially separating the histidine-linked conjugates from the remaining reactants.
Claims39
A composition comprising protein-polymer conjugates, in which a majority of the conjugates comprise a polymer convalently conjugated to the protein only at a histidine residue.
A substantially pure protein-polymer conjugate, comprising a protein covalently conjugated to a polymer at a histidine residue on said protein, wherein said protein is an alpha interferon or IL-10.
A method of preparing protein-polymer conjugates, wherein a protein is covalently conjugated to a polymer at a histidine residue on said protein, comprising:
- a) forming a plurality of protein-polymer conjugates by reacting a protein with a sufficient amount of an activated polymer under conditions sufficient to facilitate covalent attachment of said protein to said activated polymer;
- and
- b) substantially separating the protein-polymer conjugates containing a polymer conjugated to a histidine of the protein from said plurality of protein-polymer conjugates.
Dependencies are inferred from the claim text.