Binary BAC vector
AssigneeCornell Research Foundation, Inc.
InventorCarol Marie Hamilton
Life of this patent
Estimating the term…
Abstract
The present invention is directed to a vector for transferring heterologous DNA into a plant cell. The vector is based on the bacterial artificial chromosome (BAC) vector designed for the construction of genomic libraries with large DNA inserts, and the binary (BIN) vector designed for Agrobacterium-mediated plant transformation. The BIBAC vector according to the subject invention allows the construction of plant genomic libraries with large DNA inserts that can be directly introduced into plants by transformation mediated by Agrobacterium.
Claims18
A method of making a transgenic plant transformed with a vector comprising:
- providing a vector comprising:
- a backbone which includes a first origin of replication capable of maintaining heterologous DNA as a single copy in an Escherichia coli host cell, and which further includes a second origin of replication capable of maintaining heterologous DNA as a single copy in an Agrobacterium tumefaciens host cell,
- a unique restriction endonuclease cleavage site for insertion of heterologous DNA,
- a heterologous DNA encoding a gene product inserted into the unique restriction site, and
- left and right Agrobacterium T-DNA border sequences flanking said unique restriction endonuclease cleavage site, said left and right T-DNA border sequences allowing introduction of heterologous DNA located between said left and right T-DNA border sequences into a plant cell;
- introducing said vector into plant cells;
- and propagating plants from said plant cells.
A method of introducing heterologous DNA into a plant cell comprising:
- providing a vector comprising:
- a backbone which includes a first origin of replication capable of maintaining heterologous DNA as a single copy in an Escherichia coli host cell, and which further includes a second origin of replication capable of maintaining heterologous DNA as a single copy in an Agrobacterium tumefaciens host cell,
- a unique restriction endonuclease cleavage site for insertion of heterologous DNA,
- a heterologous DNA encoding a gene product inserted into the unique restriction endonuclease cleavage site, and
- left and right Agrobacterium T-DNA border sequences flanking said unique restriction endonuclease cleavage site, said left and right T-DNA border sequences allowing introduction of heterologous DNA located between said left and right T-DNA border sequences into a plant cell;
- and introducing said vector into plant cells.
Dependencies are inferred from the claim text.