Immunomagnetic assay system for clinical diagnosis and other purposes

An automated immunomagnetic assay system for analysis of an analyte in a sample, comprising:

• (a) a reaction subsystem for reacting magnetic beads coated with an antibody, which binds to the analyte, with the sample in a solution to make a fluid sample;
• (b) a collection subsystem for capturing the magnetic beads out of the fluid sample;
• (c) a rinsing subsystem for removing non-magnetic bead debris from the collection subsystem;
• and,
• (d) a filtering subsystem for removing the captured magnetic beads from the collection subsystem and holding them for the analysis.

An automated immunomagnetic assay system for analysis of an analyte in a sample, comprising:

• (a) a reaction subsystem for reacting magnetic beads coated with an antibody, which binds to the analyte, with the sample in a solution to make a fluid sample;
• (b) a collection subsystem for capturing the magnetic beads out of the fluid sample;
• (c) a rinsing subsystem for removing non-magnetic bead debris from the collection subsystem;
• and
• (d) a filtering subsystem for removing the captured magnetic beads from the collection subsystem and holding them for the analysis;
• wherein the collection and filtering subsystems comprise:
• (i) a magnetic flow cell, the magnetic flow cell comprising
• (1) a housing;
• (2) a chamber inside the housing;
• (3) an inlet port through the housing into the chamber for flowing the fluid sample into the chamber;
• (4) an outlet port through the housing out of the chamber for flowing the fluid sample out of the chamber;
• and
• (5) a plurality of paramagnetic rods positioned inside the chamber such that the flowing fluid sample will flow past the plurality of the paramagnetic rods as it flows through the chamber;
• wherein the chamber is shaped so that its cross-sectional area expands from where it connects to the inlet port to a position past at least the plurality of the paramagnetic rods;
• and,
• (ii) a pair of movable magnets positioned such that they are movable to and from a position flanking the magnetic flow cell.

A method for capturing antibody-coated magnetic beads from a fluid sample as part of an immunomagnetic assay system, comprising the steps of:

• (a) flowing the fluid sample through a chamber, wherein the chamber comprises a plurality of paramagnetic rods positioned inside the chamber such that the flowing fluid sample flows past the plurality of the paramagnetic rods as it flows through the chamber and wherein the chamber is shaped so that its cross-sectional area expands from the fluid sample enters the chamber to a position past at least the plurality of the paramagnetic rods;
• and,
• (b) flanking the chamber with a pair of magnets as the fluid sample flows through the chamber thereby collecting the magnetic beads on the plurality of the paramagnetic rods;
• (c) rinsing non-magnetic bead debris from the chamber by flowing a buffer solution through the chamber;
• (d) removing the pair of magnets to release the magnetic particles from the plurality of the paramagnetic rods;
• and
• (e) capturing the released magnetic particles on a membrane filter provided in the chamber by reversing the flow of the buffer solution through the chamber.

A method for performing an immunomagnetic assay for an analyte in a sample, comprising the steps of:

• (a) reacting magnetic beads coated with an antibody, which binds to the analyte, with the sample in a solution to make a fluid sample;
• (b) collecting the magnetic beads out of the fluid sample by the steps of:
• (i) flowing the fluid sample through a chamber, wherein the chamber comprises a collection subsystem comprising a plurality of paramagnetic rods positioned inside the chamber such that the flowing fluid sample flows past the plurality of the paramagnetic rods as it flows through the chamber and wherein the chamber is shaped so that its cross-sectional area expands from the fluid sample enters the chamber to a position past at least the plurality of the paramagnetic rods;
• and,
• (ii) flanking the chamber with a pair of magnets as the fluid sample flows through the chamber thereby collecting the magnetic beads on the plurality of the paramagnetic rods;
• (c) rinsing non-magnetic bead debris from the collection subsystem by flowing a buffer solution through the chamber;
• (d) capturing the collected magnetic beads on a membrane filter provided in the chamber by moving the pair of magnets away from the position flanking the chamber and reverse flowing the buffer solution through the chamber;
• and
• (e) analyzing the analyte bound to the magnetic beads captured on the membrane filter.