Method of plant tissue culture and regeneration
AssigneeZeneca Limited
InventorsRobert David Hall, Franciscus Andries Krens, Henricus Adrianus Verhoeven, Maria Colijn-Hooymans, James Martin Dunwell, Guy Weyens
Life of this patent
Estimating the term…
Abstract
Plants may be regenerated from stomatal cells or protoplasts of such cells. Prior to regeneration the cells or protoplasts may be genetically transformed by the introduction of hereditary material most preferably by a DNA construct which is free of genes which specify resistance to antibiotics. The regeneration step may include callus formation on a hormone-free medium. The method is particularly suitable for sugar beet.
Claims13
A method of producing a Beta vulgaris plant, comprising culturing isolated Beta vulgaris stomatal cells in a regeneration medium and regenerating a Beta vulgaris plant from the thus cultured cells.
A method for the genetic transformation of a Beta vulgaris plant comprising introducing an hereditary material into an isolated stomatal cell of said plant or a cell population enriched in guard cells and regenerating whole plants from the transformed cell or population.
Dependencies are inferred from the claim text.