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US 5,969,211 AGranted
Pantropic retroviral vectors for gene transfer in mollusks
AssigneesTher Regents of The University of California, University of Conneticut
InventorsJane C. Burns, Thomas T. Chen
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Abstract
A method for introducing foreign nucleic acid sequences into marine mollusks. A pantropic retroviral vector containing a foreign gene sequence is introduced into fertilized mollusk embryos by electroporation. The gene sequence becomes integrated into the host DNA and encodes a functional protein product. This method has implications in the introduction of disease-resistance and growth-accelerating genes into mollusks.
Claims15
A method for introducing exogenous DNA into a germline of a marine mollusk, comprising the steps of:
- inserting said exogenous DNA into a plasmid comprising a promoter that is active in mollusks and retroviral long terminal repeats (LTRs), wherein said exogenous DNA is operably linked to said promoter, thereby generating a recombinant vector;
- forming a pseudotyped retrovirus containing VSV G protein and RNA corresponding to said recombinant vector;
- and
- infecting a mollusk embryo with said pseudotyped retrovirus so as to insert said exogenous DNA into the germline of said mollusk.
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