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US 5,969,165 AGranted
Isolation and purification of paclitaxel and other related taxanes by industrial preparative low pressure chromatography on a polymeric resin column
Assignee508037 (NB) Inc.
InventorJian Liu
Life of this patent
Estimating the term…
Abstract
A high yield and high purity method for obtaining taxane analogues from a source containing taxanes. The method employs a polymeric resin membrane for separating the analogues under low pressure without the use of complex and expensive separation/purification steps currently provided in the art.
Claims20
A method of isolating and purifying taxane analogues from a source containing taxanes, comprising:
- extracting a source of taxanes in an organic extractant;
- coating a work absorbent medium with said extractant and loading said medium in a column, said column containing an absorbent agent;
- eluting, in a first step, with an organic solvent mixture at a pressure of between 10 and 20 psi to generate fractions containing taxane compounds;
- crystallizing said fractions to provide a solid taxane compound and a mother liquor;
- concentrating said mother liquor;
- and
- eluting, in a second step, with a polar solvent mixture said mother liquor through a polymeric resin to provide at least a second taxane compound.
4 more elements
A method of isolating and purifying taxanes from a source containing taxanes, comprising:
- providing a source of said taxanes;
- extracting said taxanes from said source into an organic extraction medium to provide an organic layer containing taxane compounds;
- treating a support material with said organic layer;
- providing a low pressure column containing an absorbent agent;
- eluting, in a first step, an organic solvent through said column to elute purified taxane fractions;
- crystallizing said taxane fractions to provide a first taxane analogue and a mother liquor;
- eluting, in a second step, said mother liquor through a polymeric resin in a chromatographic column to purify and elute at least a second taxane analogue and a third taxane analogue;
- and
- collecting separated taxane analogues.
6 more elements
A method of isolating and purifying taxanes from a source containing taxanes, comprising:
- providing a source of said taxanes;
- extracting said taxanes from said source into an organic extraction medium to provide an organic layer containing taxane compounds;
- treating a support material with said organic layer;
- providing a low pressure column containing an absorbent agent;
- eluting, in a first step, at a pressure of between 10 and 20 psi, an organic solvent through said column to elute purified taxane fractions and remove flavonoid and lignan impurities;
- crystallizing said taxane fractions to provide a first taxane analogue and a mother liquor;
- eluting, in a second step, at a pressure of 30 psi, said mother liquor through a polystyrene DVB resin in a chromatographic column to purify and elute at least a second taxane analogue and a third taxane analogue;
- eluting, in a third step, said second taxane analogue and said third taxane analogue through a normal phase silica gel column to purify said second taxane analogue and said third taxane analogue;
- and
- collecting separated taxane analogues.
7 more elements
Dependencies are inferred from the claim text.