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US 5,969,129 AGranted
Purification of polynucleotides from polynucleotide/polysaccharide mixtures
AssigneeUniversity of Strathclyde
InventorsBrian Caddy, Jing Cheng
Life of this patent
Estimating the term…
Abstract
A process for isolating polynucleotides from aqueous mixtures containing both polynucleotides and polysaccharides is described. The process uses polymer gels containing --B(OH).sub.2 groups, and is useful in purifying deoxyribonucleic acid extracted from plants.
Claims17
A process for the isolation of polynucleotides from an aqueous mixture containing polynucleotides and polysaccharides, which comprises:
- a) placing the said aqueous mixture in contact with a polymer gel having free --B(OH).sub.2 groups which are reactive with the polysaccharides and substantially non-reactive with the polynucleotides, wherein upon reaction with the polysaccharide the polymer gel forms a solid or semi-solid material containing the polysaccharides;
- and
- b) separating the polynucleotides from said solid or semi-solid polysaccharide material by centrifugation on the basis of said solid or semi-solid polysaccharide-containing material having a density different from the polynucleotide-containing solution.
A process for the isolation of RNA from an aqueous mixture containing RNA and polysaccharides, which comprises:
- a) placing said aqueous mixture in contact with a polymer gel having free --B(OH).sub.2 groups which are reactive with the polysaccharides, wherein upon reaction with polysaccharide, the polymer gel forms a solid or semi-solid material containing the polysaccharides;
- and
- b) separating the RNA from said solid or semi-solid polysaccharide-containing material.
Dependencies are inferred from the claim text.