3' untranslated regions of .alpha.-amylase genes
AssigneeAcademia Sinica
InventorsSu-May Yu, Ming-Tsair Chan
Life of this patent
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Abstract
An expression vector comprising a promoter capable of directing expression of a coding sequence in an angiosperm cell, and a 3' untranslated region of a rice .alpha.-amylase gene, wherein, after the coding sequence is inserted into the vector, the 3' untranslated region and the coding sequence are transcribed into a single mRNA. Also featured are methods of producing a polypeptide in angiosperm cells using such a vector.
Claims18
An expression vector comprising a promoter capable of directing expression of a coding sequence in an angiosperm cell, and a 3' untranslated region of a rice .alpha.-amylase gene, wherein, after the coding sequence is inserted into the vector, the 3' untranslated region and the coding sequence are transcribed into a single mRNA whereby the 3' untranslated region of the rice .alpha.-amylase gene increases stability of the mRNA.
A method of producing a polypeptide in an angiosperm cell, said method comprising:
- providing an expression construct which comprises (i) a coding sequence for the polypeptide, (ii) a promoter capable of directing expression of the coding sequence in the angiosperm cell, and (iii) a 3' untranslated region of a rice .alpha.-amylase gene, wherein the coding sequence and the untranslated region are heterologous to each other and positioned for transcription into a single mRNA whereby the 3' untranslated region of the rice .alpha.-amylase gene increases stability of the mRNA;
- transforming an angiosperm host cell with the expression construct;
- and
- subjecting the transformant host cell to a sugar-free environment to promote expression of the polypeptide.
A method of producing a polypeptide in an angiosperm cell, said method comprising:
- providing an expression construct which comprises (i) a coding sequence for the polypeptide, (ii) a promoter capable of directing expression of the coding sequence in the angiosperm cell, and (iii) a 3' untranslated region of a rice .alpha.-amylase gene, wherein the coding sequence and the untranslated region are heterologous to each other and positioned for transcription into a single mRNA whereby the 3' untranslated region of the rice .alpha.-amylase gene increases stability of the mRNA;
- transforming an angiosperm host cell with the construct;
- and
- subjecting the transformant host cell to a sugar-containing environment to promote expression of the polypeptide.
Dependencies are inferred from the claim text.