GNRH-leukotoxin chimeras
AssigneeUniversity of Saskatchewan
InventorsAndrew A. Potter, John G. Manns
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Abstract
New immunological carrier systems, DNA encoding the same, and the use of these systems, are disclosed. The carrier systems include chimeric proteins which comprise a leukotoxin polypeptide fused to a selected GnRH multimer which consists essentially of at least one repeating GnRH decapeptide sequence, or at least one repeating unit of a sequence corresponding to at least one epitope of a selected GnRH molecule. Under the invention, the selected GnRH sequences may all be the same, or may correspond to different derivatives, analogues, variants or epitopes of GnRH so long as the GnRH sequences are capable of eliciting an immune response. The leukotoxin functions to increase the immunogenicity of the GnRH multimer fused thereto.
Claims21
A DNA construct encoding a chimeric protein comprising a leukotoxin polypeptide fused to a multimer having more than one selected gonadotropin releasing hormone (GnRH) polypeptide, said DNA construct comprising a first nucleotide sequence encoding a leukotoxin polypeptide operably linked to a second nucleotide sequence encoding a GnRH multimer.
A DNA construct encoding a chimeric protein, wherein the chimeric protein comprises a leukotoxin polypeptide fused to first and second multimers wherein the C-terminus of the first multimer is fused to the N-terminus of the leukotoxin polypeptide and the N-terminus of the second multimer is fused to the C-terminus of the leukotoxin polypeptide, and further wherein each of said multimers comprises more than one selected gonadotropin releasing hormone (GnRH) polypeptide, said DNA construct comprising:
- a first nucleotide sequence encoding the first GnRH multimer;
- and
- a second nucleotide sequence encoding the second GnRH multimer;
- wherein said first and second nucleotide sequences are operably linked by a third nucleotide sequence encoding a leukotoxin polypeptide.
A DNA construct encoding a chimeric protein comprising a leukotoxin polypeptide fused to a multimer having eight selected gonadotropin releasing hormone (GnRH) polypeptides, wherein the C-terminus of the leukotoxin polypeptide is fused to the N-terminus of the multimer.
A DNA construct encoding a chimeric protein comprising a leukotoxin polypeptide fused to a multimer having eight selected gonadotropin releasing hormone (GnRH) polypeptides, wherein the C-terminus of the multimer is fused to the N-terminus of the leukotoxin polypeptide.
Dependencies are inferred from the claim text.