Antibodies that bind hek ligands
AssigneeImmunex Corporation
InventorsM. Patricia Beckmann, Douglas P. Cerretti
Life of this patent
Estimating the term…
Abstract
Antibodies specific for a hek-L may be generated, using a hek-L polypeptide or fragment thereof as an immunogen. The antibodies may be monoclonal.
Claims23
A purified antibody that is immunoreactive with a hek ligand (hek-L) protein, wherein the hek-L protein is selected from the group consisting of the protein of SEQ ID NO:2 and the protein of SEQ ID NO:4.
A purified antibody that is specific for a hek-L protein, wherein said hek-L protein comprises the amino acid sequence presented as residues 1 to 219 of SEQ ID NO:2.
A purified antibody that is specific for a hek-L protein, wherein said hek-L protein comprises the amino acid sequence presented as residues 1 to 202 of SEQ ID NO:2.
A purified antibody that is specific for a hek-L protein, wherein said hek-L protein comprises the amino acid sequence presented as residues 1 to 179 of SEQ ID NO:4.
A purified antibody that is specific for a hek-L protein, wherein said hek-L protein comprises the amino acid sequence presented as residues 1 to 160 of SEQ ID NO:4.
A method for producing a monoclonal antibody specific for a hek ligand (hek-L) polypeptide, said method comprising the steps of:
- a) immunizing an animal with a hek-L polypeptide selected from the group consisting of the protein of SEQ ID NO:2, an immunogenic fragment of the protein of SEQ ID NO:2, the protein of SEQ ID NO:4, and an immunogenic fragment of the protein of SEQ ID NO:4;
- b) harvesting spleen cells from the immunized animal;
- c) fusing said spleen cells to a myeloma cell line, thereby generating hybridoma cells;
- d) identifying a hybridoma cell line that produces monoclonal antibodies specific for the hek-L polypeptide;
- and
- e) purifying monoclonal antibodies produced by said hybridoma cell line.
A method of producing a hybridoma cell line, said method comprising the steps of:
- a) immunizing an animal with a hek-L polypeptide selected from the group consisting of the protein of SEQ ID NO:2, an immunogenic fragment of the protein of SEQ ID NO:2, the protein of SEQ ID NO:4, and an immunogenic fragment of the protein of SEQ ID NO:4;
- b) harvesting spleen cells from said animal;
- c) fusing said spleen cells to a myeloma cell line, thereby generating hybridoma cells;
- and
- d) identifying a hybridoma cell line that produces a monoclonal antibody specific for the hek-L polypeptide.
A method for producing an antibody, comprising immunizing an animal with a hek-L polypeptide selected from the group consisting of the protein of SEQ ID NO:2, an immunogenic fragment of the protein of SEQ ID NO:2, the protein of SEQ ID NO:4, and an immunogenic fragment of the protein of SEQ ID NO:4;
- whereby antibodies directed against the hek-L polypeptide are generated in said animal.
Dependencies are inferred from the claim text.