Secreted proteins
AssigneeGenetics Institute, Inc.
InventorsKenneth Jacobs, Kerry Kelleher, McKeough Carlin, John M. McCoy
Life of this patent
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Abstract
Novel polynucleotides and the proteins encoded thereby are disclosed.
Claims16
A protein produced according to a process comprising the following steps:
- (a) growing a culture of a host cell in a suitable culture medium;
- and
- (b) purifying the protein from the culture;
- wherein said host cell is transformed with an isolated polynucleotide operably linked to an expression control sequence, and wherein said polynucleotide is selected from the group consisting of:
- (i) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:1 from nucleotide 38 to nucleotide 1447;
- and
- (ii a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:2.
A composition comprising a protein, wherein said protein comprises the amino acid sequence of SEQ ID NO:2 the protein being substantially free from other mammalian proteins.
A protein produced according to a process comprising the following steps:
- (a) growing a culture of a host cell in a suitable culture medium;
- and
- (b) purifying the protein from the culture;
- wherein said host cell is transformed with an isolated polynucleotide operably linked to an expression control sequence, and wherein said polynucleotide is selected from the group consisting of:
- (i) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:3 from nucleotide 52 to nucleotide 2034;
- and
- (ii) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:4.
A composition comprising a protein, wherein said protein comprises the amino acid sequence of SEQ ID NO:4 the protein being substantially free from other mammalian proteins.
A protein produced according to a process comprising the following steps:
- (a) growing a culture of a host cell in a suitable culture medium;
- and
- (b) purifying the protein from the culture;
- wherein said host cell is transformed with an isolated polynucleotide operably linked to an expression control sequence, and wherein said polynucleotide is selected from the group consisting of:
- (i) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:5 from nucleotide 76 to nucleotide 474;
- and
- (ii) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:6.
A composition comprising a protein, wherein said protein comprises the amino acid sequence of SEQ ID NO:6 the protein being substantially free from other mammalian proteins.
A protein produced according to a process comprising the following steps:
- (a) growing a culture of a host cell in a suitable culture medium;
- and
- (b) purifying the protein from the culture;
- wherein said host cell is transformed with an isolated polynucleotide operably linked to an expression control sequence, and wherein said polynucleotide is selected from the group consisting of:
- (i) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:7 from nucleotide 67 to nucleotide 348;
- and
- (ii) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:8.
A composition comprising a protein, wherein said protein comprises the amino acid sequence of SEQ ID NO:8 the protein being substantially free from other mammalian proteins.
Dependencies are inferred from the claim text.