Loading patent…
AssigneeCold Spring Harbor Laboratories
InventorsDavid H. Beach, Douglas J. Demetrick, Manuel Serrano, Gregory J. Hannon
Estimating the term…
The present invention relates to the discovery in eukaryotic cells, particularly mammalian cells, of a novel family of cell-cycle regulatory proteins ("CCR-proteins"). As described herein, this family of proteins includes a polypeptide having an apparent molecular weight of 16 kDa, and a polypeptide having an apparent molecular weight of approximately 15 kDa, each of which can function as an inhibitor of cell-cycle progression, and therefore ultimately of cell growth. Thus, similar to the role of p21 to the p53 checkpoint, the subject CCR-proteins may function coordinately with the cell-cycle regulatory protein, retinoblastoma (RB). Furthermore, the CCR-protein family includes a protein having an apparent molecular weight of 13.5 kDa (hereinafter "p13.5"). The presumptive role of p13.5, like p16 and p15, is in the regulation of the cell-cycle.
An adenoviral vector comprising a nucleic acid encoding a polypeptide including an INK4 amino acid sequence which specifically binds to and inhibits a cyclin-dependent kinase (CDK), wherein said nucleic acid comprises a nucleotide sequence which hybridizes at conditions of 2.times.SSC at 50.degree. C. or higher stringency to a nucleic acid sequence of SEQ ID No:
An adenoviral vector comprising an isolated nucleic acid encoding a polypeptide including an INK4 amino acid sequence wherein said nucleic acid comprises a nucleotide sequence which hybridizes at conditions of 2.times.SSC at 50.degree. C. or higher stringency to a nucleic acid sequence of SEQ ID No:
An adenoviral vector composition comprising a nucleic acid at least 10-100 base pairs in length, which nucleic acid is complementary to and which hybridizes under conditions of 2.times.SSC at 50.degree. C. or higher stringency to sense or antisense sequence of a nucleic acid encoding a human p16 polypeptide, or naturally occurring mutants thereof wherein said human p16 polypeptide specifically binds to a cyclin dependent kinase.
A adenoviral transfection system, comprising a gene construct comprising a nucleic acid sequence which hybridizes at conditions of 2.times.SSC at 50.degree. C. or higher stringency to a nucleic acid sequence of SEQ ID No:
An adenoviral vector comprising a nucleotide sequence encoding a human p16 polypeptide, which polypeptide specifically binds a cyclin-dependent kinase (CDK).
An adenoviral vector comprising a nucleotide sequence which hybridizes under conditions of 2.times.SSC at 50.degree. C. or higher stringency to a human p16 gene and which encodes a polypeptide that specifically binds a cyclin-dependent kinase (CDK).
Dependencies are inferred from the claim text.