Glucose transporter vesicle aminopeptidase
AssigneeBayer Aktiengesellschaft
InventorsWilliam J. Knowles, Donna Guralski, Wallace Haigh, John T. Letsinger
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Abstract
An aminopeptidase which is a component of GLUT4-containing vesicles in the natural state, and which cleaves insulin. The claimed protein has a molecular weight of approximately 110 kD in its deglycosylated form. It includes the amino acid sequences Phe-Ala-Ala-Thr-Gln-Phe-Glu-Pro-Leu-Ala-Ala [SEQ ID NO: 1] and Ile-Leu-Gln-Asn-Gln-Ile-Gln-Gln-Gln-Thr-Arg-Thr-Asp-Glu-Gly-Xaa-Pro-Xaa-Me t [SEQ ID NO: 2], and reacts with antibodies produced against the peptide identified as [SEQ ID NO: 1]. Modulators of the activity of the aminopeptidase and a method for treating syndromes of insulin resistance, including diabetes, by administration of such a modulator are also claimed.
Claims4
A method for identifying modulators of GTVap activity, comprising the following steps:
- (a) providing GTVap or GTVap-containing material having an assayable amount of enzymatic activity;
- (b) incubating said GTVap or GTVap-containing material with a test substance to be assayed for ability to modulate GTVap activity;
- (c) adding a GTVap substrate;
- (d) monitoring GTVap activity as a function of time;
- and
- (e) determining the modulatory effect of said test substance on GTVap.
Dependencies are inferred from the claim text.