Keratinocyte culture from precursor cells
OwnerMarcus Baur, Thomas Hunziker +3inventors
InventorsMarcus Baur, Thomas Hunziker, Alain Limat, Wolfram Riedel, Christian Toloczyki
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Abstract
The present invention relates to the treatment of skin defect by organotypically cultured autologous keratinocytes isolated from the outer root sheath of hair follicles. Methods for primary as well as subsequent organotypic cultures (epidermal equivalents) in fully defined media eventually supplemented by autologous human serum and substances isolated from blood components, with minimal allogeneic biological supplements, are disclosed. Techniques to prepare epidermal equivalents for transplantation are included, as well as a method for the transport of the epidermal equivalents.
Claims19
A method for treating a skin defect comprising applying to said skin defect a portion of epidermal tissue comprising cultured outer root sheath cells.
A composition for treatment of a skin defect comprising differentiated outer root sheath cells formed into a dermal or an epidermal equivalent and a carrier suitable for immobilizing said dermal or epidermal equivalent within said skin defect.
A process for making a composition for healing a skit defect, comprising:
- a) primary culturing outer root sheath cells by explanting hair follicles and culturing them submerged in medium on a microporous membrane, on the undersurface of which a feeder layer of human dermal fibroblasts is placed;
- b) organotypically culturing said outer root sheath cells on a microporous membrane, on the undersurface of which a feeder layer of human dermal fibroblasts is placed, at the air-liquid interface of the medium.
A system for transporting epidermal equivalents or other skin tissue, comprising a container filled with a gelled or solid culture medium, and a carrier, wherein said epidermal equivalents or other skin tissue are fixed to said carrier and contacted with said culture medium such that said epidermal equivalents or other skin tissue are interposed between said culture medium and said carrier.
A composition for treatment of skin defects comprising a differentiated outer root sheath cells contacting a biocompatible or biodegradable matrix.
Dependencies are inferred from the claim text.