Cell separation device and in-line orifice mixer system

A method for homogeneously mixing a multi-component mixture, comprising subjecting said mixture to alternating compressional and extensional flows by forcing said components to flow together through a series of walls each wall having at least one orifice therein, wherein said walls are separated by mixing chambers, and wherein each orifice is non-aligned relative to the orifices in adjacent walls.

An in-line mixer for homogeneously mixing at least two fluids, comprising:

• a plurality of mixing chambers connected in series, each chamber being defined by a bottom wall and a side wall, wherein each said bottom wall has at least one orifice therein, and wherein a first chamber is configured to receive said at least two fluids.

A method for homogeneously mixing a multi-component mixture, comprising:

• forcing said components through a plurality of mixing chambers connected in series, said chambers being defined by a side wall and a bottom wall, said bottom wall having at least one orifice therein, wherein the fluids are accelerated from a relatively large cross-sectional area of a mixing chamber before an orifice and decelerated after passing through a relatively small cross-sectional area of said orifice into the adjacent mixing chamber.