Polynucleotide molecule from Haematococcus pluvialis encoding a polypeptide having a beta-C-4-oxygenase activity for biotechnological production of (3S, 3'S) astaxanthin and its specific expression in chromoplasts of higher plants
AssigneeYissum Research Development Company of the Hebrew University of Jerusalem
InventorsJoseph Hirschberg, Tamar Lotan
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Abstract
The present invention relates, in general, to a biotechnological method for production of (3S,3'S) astaxanthin. In particular, the present invention relates to a peptide having a .beta.-C-4-oxygenase activity; a DNA segment coding for this peptide; an RNA segments coding for this peptide; a recombinant DNA molecule comprising a vector and the DNA segment; a host cell or organism containing the above described recombinant DNA molecule or DNA segment; and to a method of biotechnologically producing (3S,3'S) astaxanthin or a food additive containing (3S,3'S) astaxanthin, using the host.
Claims17
An isolated DNA segment comprising a nucleotide sequence coding for a polypeptide having an amino acid sequence as set forth in SEQ ID NO:4 and allelic variants thereof.
An isolated RNA segment comprising a nucleotide sequence coding for a polypeptide having an amino acid sequence as set forth in SEQ ID NO:4 and allelic variants thereof.
A transgenic plant expressing a transgene including a functional portion of SEQ ID NO:1 or allelic variant thereof, said functional portion coding for a polypeptide having a .beta.-carotene C-4-oxygenase activity.
A recombinant DNA vector cormprising a first DNA segment encoding a polypeptide for directing a protein into plant chloroplasts or chromoplasts and an in frame second DNA segment including a functional portion of SEQ ID NO:1 or allelic variant thereof, said functional portion coding for a polypeptide have a .beta.-carotene C-4-oxygenase activity.
A recombinant DNA vector comprising a first DNA segment including a promoter highly expressible in plant chloroplasts or chromoplasts-containing tissues and a second DNA segment including a functional portion of SEQ ID NO:1 or allelic variant thereof, said functional portion coding for a polypeptide having a .beta.-carotene C-4-oxygenase activity.
Dependencies are inferred from the claim text.