Vector for introducing a gene into a plant, and methods for producing transgenic plants and multitudinously introducing genes into a plant using the vector
AssigneeNippon Paper Industries Co., Ltd.
InventorsHiroyasu Ebinuma, Koichi Sugita, Etsuko Matsunaga, Mikiko Yamakado
Life of this patent
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Abstract
A vector for introducing a desired gene into a planet, which comprises the desired gene and at least one morphological abnormally induction (MAI) gene as a marker gene, or which comprises the desired gene, at least one MAI gene and a removable element. A method for producing a transgenic plant free from the influence of a marker gene. A method for multitudinously introducing desired genes into one plant.
Claims30
A vector for introducing a desired gene into a plant, which comprises said desired gene, at least one morphological abnormality induction gene as a marker gene, and a removable DNA element, wherein said morphological abnormality induction gene is positioned such that it behaves integrally with the removable DNA element, and wherein said desired gene is positioned such that it does not behave integrally with the removable DNA element.
A method for producing a transgenic plant free from the influence of a marker gene, which comprises the following steps:
- (A) introducing a vector into a plant cell, wherein said vector comprises a desired gene, at least one morphological abnormality induction gene as a marker gene, and a removable DNA element, wherein said morphological abnormality induction gene is positioned such that it behaves integrally with the removable DNA element, and wherein said desired gene is positioned such that it does not behave integrally with the removable DNA element,
- (B) culturing the plant cell obtained in (A), detecting a morphologically abnormal plant tissue which appears during the culturing, and selecting said morphologically abnormal tissue, and
- (C) culturing said morphologically abnormal tissue selected in (B), detecting a morphologically normal tissue which appears during the culturing, and selecting said morphologically normal tissue.
A method for introducing at least two desired genes into a plant, which comprises conducting the following steps at least two times:
- (A) introducing a vector into a plant cell, wherein said vector comprises a desired gene, at least one morphological abnormality induction gene as a marker gene, and a removable DNA element, wherein said morphological abnormality induction gene is positioned such that it behaves integrally with the removable DNA element, and wherein said desired gene is positioned such that is does not behave integrally with the removable DNA element,
- (B) culturing the plant cell obtained in (A), detecting a morphologically abnormal plant tissue which appears during the culturing, and selecting said morphologically abnormal tissue, and
- (C) culturing said morphologically abnormal tissue selected in (B), detecting a morphologically normal tissue which appears during the culturing, and selecting said morphologically normal tissue.
A transgenic plant free from the influence of a marker gene produced by a method without a sexual segregation process, which comprises the following steps:
- (A) introducing a vector into a plant cell, wherein said vector comprises a desired gene, at least one morphological abnormality induction gene as a marker gene, and a removable DNA element, wherein said morphological abnormality induction gene is positioned such that it behaves integrally with the removable DNA element, and wherein said desired gene is positioned such that it does not behave integrally with the removable DNA element, and wherein said vector does not contain a gene contributing to detoxification of a plant cell growth inhibitory substance as a marker gene,
- (B) culturing the plant cell obtained in (A), detecting a morphologically abnormal plant tissue which appears during the cultivation, and selecting said morphologically abnormal tissue,
- (C) cultivating said morphologically abnormal tissue selected in (B), detecting a morphologically normal tissue which appears during the culturing, and selecting said morphologically normal tissue, and
- (D) cultivating said morphologically normal tissue to regenerate a plant.
A plant containing two or more desired genes produced by a method, which comprises the following steps:
- (A) introducing a vector into a plant cell, wherein said vector comprises a desired gene, at least one morphological abnormality induction gene as a marker gene, and a removable DNA element, wherein said morphological abnormality induction gene is positioned such that it behaves integrally with the removable DNA element, and wherein said desired gene is positioned such that it does not behave integrally with the removable DNA element, and wherein said vector does not contain a gene contributing to detoxification of a plant cell growth inhibitory substance as a marker gene,
- (B) culturing the plant cell obtained in (A), detecting a morphologically abnormal plant tissue which appears during the cultivation, and selecting said morphologically abnormal tissue,
- (C) cultivating said morphologically abnormal tissue selected in (B), detecting morphologically normal tissue which appears during the culturing, and selecting said morphologically normal tissue,
- (D) repeating steps (A) to (C) at least one time, and
- (E) cultivating said morphologically normal tissue to regenerate a plant.
A plant containing two or more desired genes produced by a method without a sexual segregation process, which comprises the following steps:
- (A) introducing a vector into a plant cell, wherein said vector comprises a desired gene, at least one morphological abnormality induction gene as a marker gene, and a removable DNA element, wherein said morphological abnormality induction gene is positioned such that it behaves integrally with the removable DNA element, and wherein said desired gene is positioned such that it does not behave integrally with the removable DNA element, and wherein said vector does not contain a gene contributing to detoxification of a plant cell growth inhibitory substance as a marker gene,
- (B) culturing the plant cell obtained in (A), detecting a morphologically abnormal plant tissue which appears during the culturing, and selecting said morphologically abnormal tissue,
- (C) culturing said morphologically abnormal tissue selected in (B), detecting morphologically normal tissue which appears during the culturing, and selecting said morphologically normal tissue,
- (D) repeating steps (A) to (C) at least one time, and
- (E) culturing said morphologically normal tissue to regenerate a plant.
Dependencies are inferred from the claim text.