Transgenic non-human mammal comprising a rabbit WAP promoter
AssigneeInstitut National de la Recherche Agronomique
InventorsLouis-Marie Houdebine, Eve Devinoy, Dominique Thepot
Life of this patent
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Abstract
A transgenic non-human mammal is provided where the genome of the mammal comprises a DNA construct comprising in operable association a rabbit WAP promoter and a DNA sequence encoding a heterologous protein. Methods of using the mammal in the production of recoverable amounts of a heterologous protein in the mammal's milk are discussed and described such that the mammal is a bio-reactor for a heterologous protein of interest. Furthermore, cells isolated from the mammal are provided for the production of a heterologous protein of interest in vitro, as well as, DNA constructs of the rabbit WAP promoter for the production of the transgenic non-human mammal.
Claims16
A transgenic non-human mammal whose genome comprises a DNA construct comprising in operable association a rabbit WAP promoter and a DNA sequence encoding a heterologous protein, wherein said rabbit WAP promoter has a length of at least 3.0 kilobases extending upstream from the transcription initiation site defined as nucleotide 1822 of the DNA sequence of the rabbit WAP gene depicted in SEQ. ID. NO:
- 2, and wherein said mammal expresses said DNA sequence such that a recoverable amount of said heterologous protein is produced in the milk of said mammal.
A process for the preparation of a heterologous protein in the milk of a female, non-human transgenic mammal comprising:
- (a) providing a female, non-human transgenic mammal whose genome comprises a DNA construct comprising in operable association a rabbit WAP promoter and a DNA sequence encoding a heterologous protein, wherein said rabbit WAP promoter has a length of at least 3.0 kilobases extending upstream from the transcription initiation site defined as nucleotide 1822 of the DNA sequence of the rabbit WAP gene depicted in SEQ. ID. NO:
- 2;
- (b) recovering milk from said female, non-human transgenic mammal;
- and
- (c) extracting said heterologous protein from said milk.
A process for the preparation of a heterologous protein in the milk of a female, non-human transgenic mammal comprising:
- (i) providing said female, non-human transgenic mammal whose genome comprises a DNA sequence comprising in operable association a rabbit WAP promoter and a DNA sequence encoding a heterologous protein, wherein said rabbit WAP promoter is the 6.3 kilobase HindIII-BamHI restriction fragment depicted in FIG. 1;
- (ii) recovering milk from said female, non-human transgenic mammal;
- and
- (iii) extracting said heterologous protein from said milk.
A process for the preparation of a heterologous protein in the milk of a female, non-human transgenic mammal comprising:
- (i) providing said female, non-human transgenic mammal whose genome comprises a DNA sequence comprising in operable association a rabbit WAP promoter and a DNA sequence encoding a heterologous protein, wherein said rabbit WAP promoter is the HindIII-EcoRI restriction fragment depicted in FIG. 1;
- (ii) recovering milk from said female, non-human transgenic mammal;
- and
- (iii) extracting said heterologous protein from said milk.
An isolated mammalian cell comprising a genome which comprises a DNA construct comprising in operable association a rabbit WAP promoter and a DNA sequence encoding a heterologous protein, wherein said rabbit WAP promoter has a length of at least 3.0 kilobases extending upstream from the transcription initiation site defined as nucleotide 1822 of the DNA sequence of the rabbit WAP gene depicted in SEQ. ID. NO:
- 2.
A DNA construct comprising in operable association a rabbit WAP promoter and a DNA sequence encoding a heterologous protein, wherein said rabbit WAP promoter has a length of at least 3.0 kilobases extending upstream from the transcription initiation site defined as nucleotide 1822 of the DNA sequence of the rabbit WAP gene depicted in SEQ. ID. NO:
- 2.
Dependencies are inferred from the claim text.