For selectable markers and promoters for plant tissue culture transformation
AssigneeThe Board of Trustees of the University of Illinois
InventorsHee-Sook Song, Jeffrey E. Brotherton, Jack M. Widholm
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Abstract
A selectable marker, the ASA2 gene of Nicotiana tabacum, is provided for transforming plant cells. The ASA2 promoter sequence is also provided which is capable of directing tissue culture specific transcription of a downstream structural gene. Also disclosed are truncated forms of the ASA2 promoter which are capable of directing high level constitutive transcription of downstream structural genes. Constructs containing the above genes and promoters are also disclosed.
Claims11
A promoter having a nucleotide sequence selected from the group consisting of a nucleotide sequence according to SEQ ID NO:
- 14 and a fragment of SEQ ID NO:
- 14 which is capable of directing the transcription of a downstream structural gene in a plant cell.
A nucleotide sequence selected from the group consisting of:
- SEQ ID NOs:
- 24, 4, 22, 14, and 25.
Dependencies are inferred from the claim text.