US 5,965,725 A · Polypeptides, and preparation and DNA encoding thereof

Abstract

The present invention relates to polypeptide having a primary structure of amino acid sequence shown by Sequence List Sequence No. 1 and DNA encoding for the polypeptide. The polypeptide is obtainable by following steps (1)-(3): Step (1): extracting small granule fraction of homocytes of horseshoe crab with a buffer containing protein denaturing agent and chelating agent, Step (2): subjecting said extract to reverse phase high performance liquid chromatography, Step (3): eluting by concentration gradient elution with a hydrophobic organic solvent. Also, the polypeptide is produced by chemical synthesis. The polypeptide has similar chemical structure to defensin and is useful as gargles, disinfectants, antiseptics or antimicrobials.

Claims3

Dependent claims

A single stranded DNA comprising a nucleotide sequence encoding the polypeptide shown by Formula 3 (SEO ID NO:1):

(Formula 3(SEQ ID NO:1)) Asn Pro Leu Ile Pro Ala Ile Tyr Ile Gly Ala Thr Val 1 5 10 - Gly Pro Ser Val Trp Ala Tyr Leu Val Ala Leu Val Gly 15 20 25 - Ala Ala Ala Val Thr Ala Ala Asn Ile Arg Arg Ala Ser 30 35 - Ser Asp Asn His Ser Cys Ala Gly Asn Arg Gly Trp Cys 40 45 50 - Arg Ser Lys Cys Phe Arg His Glu Tyr Val Asp Thr Tyr 55 60 65 - Tyr Ser Ala Val Cys Gly Arg Tyr Phe Cys Cys Arg Ser 70 75 - Arg
wherein, cysteine residues at 45th, 52nd, 56th, 70th, 75th and 76th positions may form disulfide bond (--S--S--) between at least one combination of 45th and 76th, 52nd and 70th, and 56th and 75th positions.

Dependencies are inferred from the claim text.

Polypeptides, and preparation and DNA encoding thereof

Life of this patent

Polypeptides, and preparation and DNA encoding thereof

Life of this patent

Abstract

Claims3