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AssigneeMatsushita Electric Industrial Co., Ltd.
InventorsNobuyuki Shigeto, Jinsei Miyazaki, Hiroshi Nakayama
Estimating the term…
It is intended to present a sensor capable of labeling with more dyes, applying the labeled protein in immunochromatography making use of antigen-antibody reaction, and having an excellent sensitivity. In a buffer, a protein and a first covalent bonding compound that can react with this protein are reacted to prepare a protein conjugate, then a cyanine labeling dye is added in the buffer containing the protein conjugate, and the protein conjugate and cyanine labeling dye are reacted to prepare a dye labeled protein conjugate. Alternatively, in a buffer solution, a protein and a cyanine labeling dye are reacted to prepare a dye labeled protein, then a first covalent bonding compound that can react with the protein is added in the buffer containing the dye labeled protein, and the dye labeled protein and first covalent bonding protein are reacted to prepare a dye labeled protein conjugate.
A dye labeled protein conjugate comprising:
A dye labeled protein conjugate comprising:
A method of preparing a dye labeled protein conjugate comprising:
A method of preparing a dye labeled protein conjugate comprising:
A method of preparing a dye labeled protein conjugate comprising:
A method of preparing a dye labeled protein conjugate comprising:
Dependencies are inferred from the claim text.
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No related US applications are recorded in the indexed filing history.
Production of labelled immunochemicals: with fluorescent label
Immunoglobulins [ig]
Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof: chromogenic or luminescent peptides
Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof: involving IgG3, IgG4, IgA, or IgY
Term ended 8 Nov 2016 (estimated)
USPTO file history isn’t available for this patent, so only indexed dates are shown.
The present invention relates to a conjugate of protein labeled with a dye, its preparing method, and a sensor using the same.
Hitherto, the cyanine labeling dye having a highly reactive functional group has been often used in labeling of protein. In this case, the functional group of the labeling dye reacts with amino group or carboxyl group of protein, and about 20 to 50 molecules of labeling dye are bonded with one molecule of protein. Usually, protein contains hundreds to thousands of amino groups or carboxyl groups, but since the protein has a three-dimensional solid structure, only about 50 amino groups or carboxyl groups of them are estimated to participate in reaction. In the prior art, therefore, it was a limit to label one molecule of protein with about 50 molecules of dye.
It is hence an object of the invention (1) to enable to label with more dyes, (2) to apply labeled protein in immunochromatography utilizing antigen-antibody reaction, and (3) to obtain a sensor having an excellent sensitivity.
FIG. 1 is a preparation process diagram in an embodiment of a method of preparing dye labeled protein conjugate of the invention.
FIG. 2 is a preparation process diagram in other embodiment of a method of preparing dye labeled protein conjugate of the invention.
FIG. 3 is a preparation process diagram in a different embodiment of a method of preparing dye labeled protein conjugate of the invention.
Embodiments of the invention are described below.
A process diagram showing an embodiment of a method of preparing dye labeled protein conjugate of the invention is shown in FIG. 1. In FIG. 1, first (1) a protein and a first covalent bonding compound that can react with this protein are reacted in a buffer, and a protein conjugate is prepared. Then (2) a cyanine labeling dye expressed in chemical formula 1 is added in the buffer containing the protein conjugate, and the protein conjugate and the cyanine labeling dye are reacted. Thus, a dye labeled protein conjugate is prepared.
A process diagram showing other embodiment of a method of preparing dye labeled protein conjugate of the invention is shown in FIG. 2. In FIG. 2, first (1) a protein and a cyanine labeling dye expressed in chemical formula 1 are reacted in a buffer, and a dye labeled protein is prepared. Then (2) a first covalent bonding compound that can react with the protein is added in the buffer containing the dye labeled protein, and the dye labeled protein and first covalent bonding compound are reacted. Thus, a dye labeled protein conjugate is prepared.