DNA and encoded protein which regulates cold and dehydration regulated genes
AssigneeBoard of Trustees of Michigan State University
InventorsMichael F. Thomashow, Eric J. Stockinger
Life of this patent
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Abstract
A gene, designated as CBF1, encoding a protein, CBF1, which binds to a region regulating expression of genes which promote cold temperature and dehydration tolerance in plants is described. CBF1 is used to transform microorganisms and can be used to transform plants.
Claims20
A binding protein in isolated form comprising an amino acid sequence homologous to sufficiently homologous to an AP2 domain of SEQ. ID. No. 2 that the protein binds to a cold or dehydration transcription regulating region comprising a sequence CCG.
A binding protein in isolated form comprising an amino acid sequence sufficiently homologous to SEQ. ID. No. 2 that the protein binds to a cold or dehydration transcripion regulating region comprising a sequence CCG.
A binding protein in isolated form comprising an amino acid sequence set forth in SEQ. ID. No. 2.
A composition comprising:
- a binding protein in isolated form comprising an amino acid sequence sufficiently homologous to an AP2 domain of SEQ. ID. No. 2 that the protein binds to a cold or dehydration transcription regulations region comprising a sequence CCG;
- and
- a carrier for introducing the protein into a plant cell.
A composition comprising:
- a binding protein in isolated form comprising an amino acid sequence sufficiently homologous to SEQ. ID. No. 2 that the protein binds to a cold or dehydration transcription regulating region comprising a sequence CCG;
- and
- a carrier for introducing the protein into a plant cell.
Dependencies are inferred from the claim text.