Assay for the proteolytic activity of serotype a from clostridium botulinum
AssigneeThe United States of America as represented by the Secretary of the Army
InventorsJames J. Schmidt, Karen A. Bostian
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Abstract
A label-based assay is described, through modifications of substrate strure and derivatization of serum albumin, which can be used to determine type A proteolytic activity without separation of products.
Claims13
A substrate peptide for determining the proteolytic acitivity of botulinum neurotoxin type A using fluorescamine detection without separation of products, said substrate peptide consisting of at least 16 residues of SEQ ID NO:1, wherein said peptide is modified by acetylating the N-terminal amino group and eliminating the negative charge at the carboxy terminus.
Dependencies are inferred from the claim text.