US 5,965,510 AGranted

Enhancement of enzyme reactions

AssigneeNovo Nordisk A/S

InventorsPalle Schneider, S.o slashed.ren Ebdrup

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Abstract

This invention relates to activation of enzymes. More specifically, the invention relates to agents capable of enhancing the activity of peroxidases or peroxidase acting compounds. The invention also relates to methods of oxidizing a substrate with a source of hydrogen peroxide in the presence of a peroxidase enzyme or a peroxidase acting compound, and an enhancing agent. More specifically, the invention relates to a method of bleaching of dye in solutions, to a method of inhibiting the transfer of a textile dye from a dyed fabric to another fabric when said fabrics are washed together in a wash liquor, to a method of bleaching of lignin-containing material, in particular bleaching of pulp for paper production, to a method of treatment of waste water from pulp manufacturing, and to a method of enzymatic polymerization and/or modification of lignin or lignin containing material.

Claims34

Dependent claims

A detergent composition comprising

(a) a surfactant,
(b) an enzyme exhibiting peroxidase activity,
(c) one or more other enzymes selected from the group consisting of protease, lipase, amylase, cellulase and oxidase, and
(d) an enhancing agent of formula I ##STR9## wherein X is --O-- or --S--;
R.sup.1 -R.sup.9 independently are hydrogen, halogen, hydroxy, formyl, carboxy or an ester or salt thereof, carbamoyl, sulfo or an ester or salt thereof, sulfamoyl, phospho, phosphono, phosphonooxy or an ester or salt thereof, amino, phenyl, C.sub.1 -C.sub.14 -alkyl, C.sub.1 -C.sub.5 -alkoxy, carbonyl-C.sub.1 -C.sub.5 -alkyl, aryl-C.sub.1 -C.sub.5 -alkyl;
wherein the carbamoyl, sulfamoyl, and amino are unsubstituted or substituted once or twice with R.sup.10 ;
the phenyl is unsubstituted or substituted with one or more R.sup.10 ;
and the C.sub.1 -C.sub.14 -alkyl, C.sub.1 -C.sub.5 -alkoxy, carbonyl-C.sub.1 -C.sub.5 -alkyl, and aryl-C.sub.1 -C.sub.5 -alkyl are saturated or unsaturated, branched or unbranched, and unsubstituted or substituted with one or more R.sup.10 ;
wherein R.sup.10 is halogen, hydroxy, formyl, carboxy or an ester or salt thereof, carbamoyl, sulfo or an ester or salt thereof, sulfamoyl, phospho, phosphono, phosphonooxy or an ester or salt thereof, nitro, amino, phenyl, aminoalkyl, piperidino, piperazinyl, pyrrolidino, C.sub.1 -C.sub.5 -alkyl, C.sub.1 -C.sub.5 -alkoxy;
wherein the carbamoyl, sulfamoyl, and amino are unsubstituted or substituted once or twice with hydroxy, C.sub.1 -C.sub.5 -alkyl, C.sub.1 -C.sub.5 -alkoxy;
wherein the phenyl may be substituted with one or more of halogen, hydroxy, amino, formyl, carboxy or an ester or salt thereof, carbamoyl, sulfo or an ester or salt thereof, and sulfamoyl;
and wherein the C.sub.1 -C.sub.5 -alkyl and C.sub.1 -C.sub.5 -alkoxy are saturated or unsaturated, branched or unbranched, and may be substituted once or twice with any of halogen, hydroxy, amino, formyl, carboxy or an ester or salt thereof, carbamoyl, sulfo or an ester or salt thereof, and sulfamoyl;
or
two of the substituent groups R.sup.1 -R.sup.8 together form a group --B--, wherein B is (--CHR.sup.10 --N.dbd.N--), (--CH.dbd.CH--).sub.n, (--CH.dbd.N--).sub.n or (--N.dbd.CR.sup.10 --NR.sup.10 --) wherein n is 1, 2 or 3.

11 more elements

A method of washing a fabric, comprising treating the fabric with

(a) a surfactant,
(b) an enzyme exhibiting peroxidase activity, and
(c) one or more other enzymes selected from the group consisting of protease, lipase, amylase, cellulase and oxidase, and
(d) an enhancing agent of formula I:
##STR10## wherein X is --O-- or --S--;
R.sup.1 -R.sup.9 independently are hydrogen, halogen, hydroxy, formyl, carboxy or an ester or salt thereof, carbamoyl, sulfo or an ester or salt thereof, sulfamoyl, phospho, phosphono, phosphonooxy or an ester or salt thereof, amino, phenyl, C.sub.1 -C.sub.14 -alkyl, C.sub.1 -C.sub.5 -alkoxy, carbonyl-C.sub.1 -C.sub.5 -alkyl, aryl-C.sub.1 -C.sub.5 -alkyl;
wherein the carbamoyl, sulfamoyl, and amino are unsubstituted or substituted once or twice with R.sup.10 ;
the phenyl is unsubstituted or substituted with one or more R.sup.10 ;
and the C.sub.1 -C.sub.14 -alkyl, C.sub.1 -C.sub.5 -alkoxy, carbonyl-C.sub.1 -C.sub.5 -alkyl, and aryl-C.sub.1 -C.sub.5 -alkyl are saturated or unsaturated, branched or unbranched, and unsubstituted or substituted with one or more R.sub.10 ;
wherein R.sub.10 is halogen, hydroxy, formyl, carboxy or an ester or salt thereof, carbamoyl, sulfo or an ester or salt thereof, sulfamoyl, phospho, phosphono, phosphonooxy or an ester or salt thereof, nitro, amino, phenyl, aminoalkyl, piperidino, piperazinyl, pyrrolidino, C.sub.1 -C.sub.5 -alkyl, C.sub.1 -C.sub.5 -alkoxy;
wherein the carbamoyl, sulfamoyl, and amino are unsubstituted or substituted once or twice with hydroxy, C.sub.1 -C.sub.5 -alkyl, C.sub.1 -C.sub.5 -alkoxy;
wherein the phenyl may be substituted with one or more of halogen, hydroxy, amino, formyl, carboxy or an ester or salt thereof, carbamoyl, sulfo or an ester or salt thereof, and sulfamoyl;
and wherein the C.sub.1 -C.sub.5 -alkyl and C.sub.1 -C.sub.5 -alkoxy are saturated or unsaturated, branched or unbranched, and may be substituted once or twice with any of halogen, hydroxy, amino, formyl, carboxy or an ester or salt thereof, carbamoyl, sulfo or an ester or salt thereof, and sulfamoyl;
or
two of the substituent groups R.sup.1 -R.sup.8 together form a group --B--, wherein B is (--CHR.sup.10 --N.dbd.N--), (--CH.dbd.CH--).sub.n, (--CH.dbd.N--).sub.n or (--N .dbd.CR.sup.10 --NR.sup.10 --) wherein n is 1, 2 or 3.

12 more elements

Dependencies are inferred from the claim text.