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US 5,965,437 AGranted
Method of screening a compound for hematopoietic activity
AssigneeBeth Israel Deaconess Medical Center, Inc.
InventorDavid T. Scadden
Life of this patent
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Abstract
Methods of selecting a population of human cells containing quiescent pluripotent hematopoietic progenitor cells substantially free of mature, human myeloid and lymphoid cells, the quiescent pluripotent progenitor cells obtained by these methods, and methods of using the pluripotent progenitor cells are described.
Claims5
A method of screening a compound for hematopoietic activity comprising the steps of:
- a) contacting a population of quiescent pluripotent hematopoietic progenitor cells obtained by culturing a mammalian hematopoietic mononuclear cell suspension in the combined presence of at least one anti-metabolite agent and at least one early-acting growth factor under conditions sufficient to eliminate dividing cells and cells responsive to early-acting growth factors with a compound to be tested for hematopoietic activity and maintaining the culture under conditions sufficient for proliferation of the cells;
- and
- b) recovering the cells of step a) and evaluating the cells for differentiation characteristics whereby the presence of differentiation characteristics are indicative of the hematopoietic activity of the compound.
Dependencies are inferred from the claim text.