Method of isolating mesenchymal stem cells associated with isolated megakaryocytes by isolating megakaryocytes
AssigneeOsiris Therapeutics, Inc.
InventorsMark A. Thiede, Daniel R. Marshak
Life of this patent
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Abstract
The present invention is directed to isolated and purified human mesenchymal stem cells, to a method for isolating, purifying, and culturally expanding human mesenchymal stem cells (i.e. mesenchymal stem cells or "MSCs"), and to the characterization of and uses for such cells. In particular, the invention relates to isolation of MSCs that are associated with hematopoietic cells, such as megakaryocyte precursors, in the bone marrow.
Claims15
A process for recovering human mesenchymal stem cells, comprising:
- separating megakaryocytes from a human cell population that includes megakaryocytes and mesenchymal stem cells by use of an antibody for a surface antigen on the megakaryocytes, separated megakaryocytes having associated therewith human mesenchymal stem cells;
- and
- recovering human mesenchymal stem cells from the separated megakaryocytes.
A process for recovering human mesenchymal stem cells, comprising:
- separating megakaryocytes from a human cell population that includes megakaryocytes and mesenchymal stem cells by use of an antibody for a surface antigen on the megakaryocytes, separated megakaryocytes having associated therewith mesenchymal stem cells;
- and
- culturing the separated megakaryocytes in a culture medium that favors the growth of mesenchymal stem cells.
A process for recovering human mesenchymal stem cells comprising:
- recovering a cell population enriched for megakaryocytes from a human cell population including megakaryocytes and mesenchymal stem cells, said recovered cell population including megakaryocytes having mesenchymal stem cells associated therewith;
- separating megakaryocytes from the recovered cell population, said separated megakaryocytes including megakaryocytes having mesenchymal stem cells associated therewith;
- and
- recovering human mesenchymal stem cells from the separated megakaryocytes.
Dependencies are inferred from the claim text.