Methods for making neisseria or hemophilus IgA protease and DNA encoding the proteases
AssigneeBoehringer Mannheim GmbH
InventorsDorothea Ambrosius, Carola Dony, Rainer Rudolph
Life of this patent
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Abstract
The present invention concerns a process for the isolation of recombinant IgA protease from inclusion bodies. In addition a recombinant DNA is claimed which codes for an IgA protease whose C-terminal helper sequence and preferably also its N-terminal signal sequence is no longer active.
Claims15
The method for producing a recombinant Neisseria or Haemophilus IgA protease, comprising:
- (a) modifying a gene which codes for a Neisseria or Haemophilus IgA protein to inactivate a C-terminal helper sequence of said Neisseria or Haemophilus IgA protease,
- (b) transforming a prokaryotic host cell with a DNA molecule which comprises the modified gene of (a),
- (c) culturing the prokaryotic host cell transformed in (b) to express recombinant Neisseria or Haemophilus IgA protease as inclusion bodies,
- (d) isolating inclusion bodes formed in (c), and
- (e) converting isolated inclusion bodies into active Neisseria or Haemophilus IgA protease.
An isolated DNA molecule which codes for a Neisseria or Haemophilus IgA protease which has an inactive C-terminal helper sequence and an inactive N-terminal signal sequence.
An isolated DNA molecule which codes for a Neisseria or Haemophilus IgA protease which lacks both a C-terminal helper sequence and an N-terminal helper sequence.
Dependencies are inferred from the claim text.