Purified sulfotransferase
AssigneeTakara Shuzo Co., Ltd.
InventorKoichi Honke
Life of this patent
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Abstract
An isolated gene encoding a polypeptide possessing sulfotransferase activity which specifically transfers a sulfate group to the C-3-position hydroxyl group of Gal by acting on a sugar chain represented by Gal.beta.1-R, wherein Gal represents galactose; R represents a carbohydrate, lipid or glycoconjugate, and a method for producing the polypeptide.
Claims6
A purified polypeptide having sulfotransferase activity that specifically transfers a sulfate group to the C-3 position hydroxyl group of galactose by acting on a sugar chain represented by Gal.beta.1-R, wherein Gal represents galactose and R represents a carbohydrate, lipid or glycoconjugate, wherein said polypeptide is encoded by a DNA molecule being selected from the group consisting of
- a) a DNA molecule comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 1,
- b) a DNA molecule comprising the nucleotide sequence of SEQ ID NO:
- 2, and
- c) a DNA molecule that hybridizes to the DNA molecule of a) or b), or the complement thereof, at a stringency equivalent to 6.times. SSC, 0.5% sodium dodecyl sulfate, and 5.times. Denhardt's at 50.degree. C.
Dependencies are inferred from the claim text.