Arabidepsi thaliana proteins having .DELTA.-5,7-sterol-.DELTA.-7-reductase activity
AssigneeRoussel Uclaf
InventorsXavier Chenivesse, Catherine Duport, Eric Lecain, Denis Pompon
Life of this patent
Estimating the term…
Abstract
A nucleic acid sequence containing a sequence coding for a protein having a .DELTA.-5,7 sterol, .DELTA.-7 reductase activity said nucleic acid being a DNA or an RNAg production process, strains of transformed yeasts and uses thereof
Claims6
An Arabidepsi thaliana protein having a .DELTA.-5,7 sterol, .DELTA.-7 reductase activity and having the amino acid sequence of SEQ ID No:
- 2:
- Met Ala Glu Thr Val His Ser Pro Ile Val Thr Tyr 1 5 10 Ala Ser Met Leu Ser Leu Leu Ala Phe Cys Pro Pro 15 20 Phe Val Ile Leu Leu Trp Tyr Thr Met Val His Gln 25 30 35 Asp Gly Ser Val Thr Gln Thr Phe Gly Phe Phe Trp 40 45 Glu Asn Gly Val Gln Gly Leu Ile Asn Ile Trp Pro 50 55 60 Arg Pro Thr Leu Ile Ala Trp Lys Ile Ile Phe Cys 65 70 Tyr Gly Ala Phe Glu Ala Ile Leu Gln Leu Leu Leu 75 80 Pro Gly Lys Arg Val Glu Gly Pro Ile Ser Pro Ala 85 90 95 Gly Asn Arg Pro Val Tyr Lys Ala Asn Gly Leu Ala 100 105 Ala Tyr Phe Val Thr Leu Ala Thr His Leu Gly Leu 110 115 120 Trp Trp Phe Gly Ile Phe Asn Pro Ala Ile Val Tyr 125 130 Asp His Leu Gly Glu Ile Phe Ser Ala Leu Ile Phe 135 140 Gly Ser Phe Ile Phe Cys Val Leu Leu Tyr Ile Lys 145 150 155 Gly His Val Ala Pro Ser Ser Ser Asp Ser Gly Ser 160 165 Cys Gly Asn Leu Ile Ile Asp Phe Tyr Trp Gly Met 170 175 180 Glu Leu Tyr Pro Arg Ile Gly Lys Ser Phe Asp Ile 185 190 Lys Val Phe Thr Asn Cys Arg Phe Gly Met Met Ser 195 200 Trp Ala Val Leu Ala Val Thr Tyr Cys Ile Lys Gln 205 210 215 Tyr Glu Ile Asn Gly Lys Val Ser Asp Ser Met Leu 220 225 Val Asn Thr Ile Leu Met Leu Val Tyr Val Thr Lys 230 235 240 Phe Phe Trp Trp Glu Ala Gly Tyr Trp Asn Thr Met 245 250 Asp Ile Ala His Asp Arg Ala Gly Phe Tyr Ile Cys 255 260 Trp Gly Cys Leu Val Trp Val Pro Ser Val Tyr Thr 265 270 275 Ser Pro Gly Met Tyr Leu Val Asn His Pro Val Glu 280 285 Leu Gly Thr Gln Leu Ala Ile Tyr Ile Leu Val Ala 290 295 300 Gly Ile Leu Cys Ile Tyr Ile Lys Tyr Asp Cys Asp 305 310 Arg Gln Arg Gln Glu Phe Arg Arg Thr Asn Gly Lys 315 320 Cys Leu Val Trp Gly Arg Ala Pro Ser Lys Ile Val 325 330 335 Ala Ser Tyr Thr Thr Thr Ser Gly Glu Thr Lys Thr 340 345 Ser Leu Leu Leu Thr Ser Gly Trp Trp Gly Leu Ala 350 355 360 Arg His Phe His Tyr Val Pro Glu Ile Leu Ser Ala 365 370 Phe Phe Trp Thr Val Pro Ala Leu Phe Asp Asn Phe 375 380 Leu Ala Tyr Phe Tyr Val Leu Thr Leu Leu Leu Phe 385 390 395 Asp Arg Ala Lys Arg Asp Asp Asp Arg Cys Arg Ser 400 405 Lys Tyr Gly Lys Tyr Trp Lys Leu Try Cys Glu Lys 410 415 420 Val Lys Tyr Arg Ile Ile Pro Gly Ile Try 425 430
- and the allelic variants and analogs of this sequence having enzylmatic activity.
An Arabidepsis thaliana protein having a .DELTA.-5,7 sterol, .DELTA.-7 reductase activity and having the amino acid sequence of SEQ ID No:
- 2 and designated .DELTA.-7Red.
A protein having a .DELTA.-5,7 sterol, .DELTA.-7 reductase activity obtained by expression in a host cell containing a DNA sequence coding for the said protein.
An A. thaliana protein having a .DELTA.-5,7 sterol, .DELTA.-7 reductase activity obtained by expression in a host cell containing a DNA sequence coding for the amino acid sequence of SEQ ID No:
- 2.
Dependencies are inferred from the claim text.