Arginyl tRNA synthetase from staphyloccus aureus
AssigneeSmithKline Beecham Corporation
InventorsJohn Edward Hodgson, Elizabeth Jane Lawlor
Life of this patent
Estimating the term…
Abstract
The invention provides tRNA synthetase polypeptides and DNA (RNA) encoding tRNA synthetase polypetides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing tRNA synthetase polypeptide for the protection against infection, particularly bacterial infections.
Claims21
An isolated polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:2.
An isolated polypeptide comprising a polypeptide sequence selected from the group consisting of:
- (a) a first sequence which is SEQ ID NO:2,
- (b) a second sequence comprising a truncation of the first sequence containing at least 30 amino acids,
- (c) a third sequence comprising a truncation of the first sequence containing at least 50 amino acids,
- (d) a fourth sequence which is identical to the first sequence except that the fourth sequence has 1-5 mutations relative to the first sequence, wherein each mutation is a substitution, deletion or insertion of one amino acid, or
- (e) a fifth sequence which is identical to the first sequence except that the fifth sequence has 5-10 mutations relative to the first sequence, wherein each mutation is a substitution, deletion or insertion of one amino acid,
- wherein the isolated polypeptide has tRNA synthetase enzymatic activity.
An isolated polypeptide encoded by an isolated first polynucleotide wherein the isolated first polynucleotide hybridizes under stringent conditions to a second polynucleotide which encodes the mature polypeptide of SEQ ID NO:2;
- wherein stringent conditions comprise overnight incubation at 42.degree. C. in a solution comprising:
- 50% formamide, 5.times.SSC (150 mM NacL, 15 mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5.times. Denhardt's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing the filters in 0.1.times. SSC at about 65.degree. C.;
- wherein the isolated polypeptide is tRNA synthetase polypeptide expressed by the DNA contained in NCIMB Deposit No. 40771.
An isolated polypeptide encoded by an isolated first polynucleotide wherein the isolated first polynucleotide hybridizes under stringent conditions to the polynucleotide sequence of SEQ ID NO:1, wherein stringent conditions comprise overnight incubation at 42.degree. C. in a solution comprising:
- 50% formamide, 5.times.SSC (150 mM NacL, 15 mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5.times. Denhardt's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing the filters in 0.1.times. SSC at about 65.degree. C., wherein the isolated polypeptide comprises a sequence of at least 30 amino acids.
An isolated polypeptide comprising a polypeptide sequence selected from the group consisting of:
- (a) a first sequence which is SEQ ID NO:2,
- (b) a second sequence comprising a truncation of the first sequence containing at least 30 amino acids,
- (c) a third sequence comprising a truncation of the first sequence containing at least 50 amino acids,
- (d) a fourth sequence which is identical to the first sequence except that the fourth sequence has 1-5 mutations relative to the first sequence, wherein each mutation is a substitution, deletion or insertion of one amino acid, or
- (e) a fifth sequence which is identical to the first sequence except that the fifth sequence has 5-10 mutations relative to the first sequence, wherein each mutation is a substitution, deletion or insertion of one amino acid,
- wherein the isolated polypeptide is capable of generating antibodies having binding specificity for the amino acid sequence set forth in SEQ ID NO:2.
Dependencies are inferred from the claim text.