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US 5,965,414 AGranted
Process for producing yeast and mold inhibiting products with lactobacillus
AssigneeMicrolife Technics, Inc.
InventorsPeter A. Vandenbergh, Stephen W. King
Life of this patent
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Abstract
A method for producing a novel yeast and mold inhibiting products (FIC) from a Lactobacillus, particularly a Lactobacillus casei having the yeast and mold producing characteristics of Lactobacillus casei var. rhamnosus NRRL-B-15972 is described. The products (FIC) are particularly useful in retarding yeast and mold growth in foods and other materials in need thereof.
Claims23
A process for producing yeast and mold growth inhibiting products (FIC) which comprises:
- (a) incubating live cells of Lactobacillus having all of the identifying characteristics of Lactobacillus casei var. rhamnosus NRRL-B-15972 in a nutrient medium for the cells containing effective amounts of a sulfur containing organic compound which induce the formation of the products (FIC) and of a carbon source so as to produce an isolatable amount of the products (FIC) in the nutrient medium;
- and
- (b) recovering the products (FIC) from the nutrient medium, wherein the products (FIC) are polar, non-protein, non-lipid, have a molecular size of less than 1000 daltons, insoluble in chloroform or hexane and soluble in ethanol, in butanol and acetone, are temperature stable at between -70.degree. C. and 100.degree. C. and are stable at a pH between 3 to 10.
A process for producing yeast and mold growth inhibiting products (FIC) which comprises:
- (a) incubating live cells of a Lactobacillus having all of the identifying characteristics of Lactobacillus casei var. rhamnosus NRRL-B-15972 in a nutrient medium for the cells containing effective amounts of growth factors present in a composition selected from the group consisting of cysteine, garlic extract, milk, whey, yeast, a yeast extract, molasses and a protein digest which induce the formation of the products (FIC), of a protein source and of a carbon source, so as to produce the products (FIC) in the nutrient medium;
- and
- (b) treating the growth medium which has been incubated so as to recover isolatable amounts of the products (FIC);
- and
- (c) recovering the products FIC from the nutrient medium, wherein the products (FIC) are polar, non-protein, non-lipid, have a molecular size of less than 1000 daltons, are insoluble in chloroform or hexane and soluble in ethanol, in butanol and acetone, are temperature stable at between -70.degree. C. and 100.degree. C. and are stable at a pH between 3 to 10.
2 more elements
A process for producing yeast and mold growth inhibiting products (FIC) which comprises:
- (a) incubating live cells of a Lactobacillus having all of the identifying characteristics of Lactobacillus casei var. rhamnosus NRRL-B-15972 in a nutrient medium for the cells containing effective amounts of growth factors present in a composition selected from the group consisting of cysteine, garlic extract, milk, whey, yeast, a yeast extract, molasses and a protein digest which induce the formation of the products (FIC), a protein source and of a carbon source to thereby produce isolatable amounts of products (FIC) in the nutrient medium;
- and
- (b) treating the nutrient medium which has been incubated to recover the products (FIC) with removal of water from the nutrient medium to produce the products (FIC), wherein the products (FIC) are polar, non-protein, non-lipid, have a molecular size of less than 1000 daltons, are insoluble in chloroform or hexane and soluble in ethanol, in butanol and acetone, are temperature stable at between -70.degree. C. and 100.degree. C. and are stable at a pH between 3 to 10.
A process for producing yeast and mold growth inhibiting products (FIC) which comprises:
- (a) incubating cells of a Lactobacillus having all of the identifying characteristics of Lactobacillus casei var. rhamnosus NRRL-B-15972 in a nutrient medium for the cells containing effective amounts of growth factors present in compositions selected from the group consisting of cysteine, garlic extract, milk, whey, yeast, a yeast extract, molasses and a protein digest which induce the formation of the products (FIC) and of a carbon source so as to produce the products (FIC) in the nutrient medium;
- (b) mixing the incubated nutrient medium with an organic solvent which extracts the products (FIC) into the solvent;
- (c) separating the solvent with the extracted products (FIC) from the nutrient medium;
- and
- (d) separating the products (FIC) from the solvent, wherein the products (FIC) are polar, non-protein, non-lipid, have a molecular size of less than 1000 daltons, are insoluble in chloroform or hexane and soluble in ethanol, in butanol and acetone, are temperature stable at between -70.degree. C. and 100.degree. C. and are stable at a pH between 3 to 10.
2 more elements
A process for producing fungal growth inhibiting products (FIC) which comprises:
- (a) incubating cells of a Lactobacillus having all of the identifying characteristics of Lactobacillus casei var. rhamnosus NRRL-B-15972 in a nutrient medium for the cells containing effective amounts of growth factors present in cysteine, garlic extract, milk, whey, yeast, yeast extract, molasses or protein digest which induce the formation of the products (FIC), of a protein source and of a carbon source so as to produce the products (FIC) in the nutrient medium;
- and
- (b) recovering the products (FIC) from the nutrient medium and cells, wherein the products (FIC) are polar, non-protein, non-lipid, have a molecular size of less than 1000 daltons, are insoluble in chloroform or hexane and soluble in ethanol, in butanol and acetone, are temperature stable at between -70.degree. C. and 100.degree. C. and are stable at a pH between 3 to 10.
A process for producing fungal growth inhibiting products (FIC) which comprises:
- (a) incubating live cells of a Lactobacillus having all of the identifying characteristics of Lactobacillus casei var. rhamnosus NRRL-B-15972 in a nutrient medium containing effective amounts of growth factors for the cells which stimulate the cells to produce an isolatable amount of the products (FIC) in the nutrient medium;
- and
- (b) recovering the products (FIC) from the nutrient medium, wherein the products (FIC) are polar, non-protein, non-lipid, have a molecular size of less than 1000 daltons, are insoluble in chloroform or hexane and soluble in ethanol, in butanol and acetone, are temperature stable at between -70.degree. C. and 100.degree. C. and are stable at a pH between 3 to 10.
Dependencies are inferred from the claim text.