Kojibiose phosphorylase obtainable from Thermoanaerobium brockii, its preparation and uses
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsTomoyuki Nishimoto, Michio Kubota, Hiroto Chaen, Toshio Miyake
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Abstract
A kojibiose phosphorylase which hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form .beta.-glucose and .beta.-D-glucose-1-phosphoric acid, forms kojibiose and an inorganic phosphoric acid from .beta.-D-glucose-1-phosphoric acid, and catalyzes the transfer reaction of glucosyl group to other saccharides using .beta.-D-glucose-1-phosphoric acid as a saccharide donor. The enzyme is obtainable from Thermoanaerobium brockii and obtainable by recombinant technology.
Claims12
A purified or isolated kojibiose phosphorylase obtainable from Thermoanaerobium brockii which catalyzes the phosphorolysis of kojibiose in the presence of inorganic phosphoric acid and/or its salt to form D-glucose and .beta.-D-glucose-1-phosphoric acid and/or its salt.
A purified or isolated kojibiose phosphorylase, which has the following physicochemical properties:
- (1) Molecular weight of 83,000.+-.5,000 daltons on SDS-polyacrylamide gel electrophoresis (SDS-PAGE);
- (2) Optimum temperature at about 65.degree. C. when incubated at pH 5.5 for 30 min;
- (3) Optimum pH at about 5.5 when incubated at 60.degree. C. for 30 min;
- (4) Stable up to a temperature of about 65.degree. C. when incubated at pH 5.5 for one hour;
- and
- (5) Stable at pH values of about 5.5-10.0 when incubated at 4.degree. C. for 24 hours.
Dependencies are inferred from the claim text.