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US 5,965,405 AGranted
Method for producing Fv fragments in eukaryotic cells
AssigneeCelltech Limited
InventorsGregory Paul Winter, Lutz Riechmann, Geoffrey Thomas Yarranton, Mark William Bodmer, Raymond John Owens
Life of this patent
Estimating the term…
Abstract
Methods for preparing Fv fragments which lack linking polypeptides in eukaryotic cells are provided.
Claims10
A method for producing Fv fragments comprising:
- (a) providing a first transcription unit which encodes a signal sequence 5' to a first DNA and operatively linked to said first DNA, and wherein said first DNA encodes the variable domain only of an antibody heavy chain;
- (b) providing a second transcription unit which encodes a signal sequence 5' to a second DNA and operatively linked to said second DNA, and wherein said second DNA encodes the variable domain only of an antibody light chain, said light chain variable domain being complementary to the heavy chain variable domain;
- (c) inserting the first transcription unit into a first eukaryotic expression vector;
- (d) inserting the second transcription unit into a second eukaryotic expression vector;
- (e) transforming a eukaryotic host cell with both the first and second expression vectors;
- and
- (f) culturing the transformed host cell under conditions which cause the heavy and light chain variable domains to be synthesized, correctly assembled and secreted by the host cell such that functional Fv fragments are produced in the culture supernatant.
4 more elements
A method for producing Fv fragments comprising:
- (a) providing a first transcription unit which encodes a signal sequence 5' to a first DNA and operatively linked to said first DNA, wherein said first DNA encodes the variable domain only of an antibody heavy chain;
- (b) providing a second transcription unit which encodes a signal sequence 5' to a second DNA and operatively linked to said second DNA, wherein said second DNA encodes the variable domain only of an antibody light chain, the light chain variable domain being complementary to the heavy chain variable domain;
- (c) inserting the first transcription unit into a eukaryotic expression vector;
- (d) inserting the second transcription unit into said eukaryotic expression vector;
- (e) transforming a eukaryotic host cell with the eukaryotic expression vector containing both the first and second transcription units;
- and,
- (f) culturing the transformed host cell under conditions which cause the heavy and light chain variable domains to be synthesized, correctly assembled and secreted by the host cell such that functional Fv fragments are produced in the culture supernatant.
4 more elements
A method for producing Fv fragments comprising:
- providing a first eukaryotic expression vector which comprises a signal sequence 5' to a first DNA and operatively linked to said first DNA, wherein said first DNA sequence encodes the variable domain only of an antibody heavy chain;
- providing a second eukaryotic expression vector which comprises a signal sequence 5' to a second DNA and operatively linked to said second DNA, wherein said second DNA encodes the variable domain only of an antibody light chain, said light chain variable domain being complementary to the heavy chain variable domain;
- transforming a eukaryotic host cell with both the first and second expression vectors;
- and
- culturing the transformed host cell under conditions which cause the heavy and light chain variable domains to be synthesized, correctly assembled and secreted by the host cell, such that functional Fv fragments are produced in the culture supernatant.
2 more elements
A method for producing Fv fragments comprising the steps:
- (a) providing a eukaryotic expression vector which comprises:
- a signal sequence 5' to a first DNA and operatively linked to said first DNA, wherein said first DNA encodes the variable domain only of an antibody heavy chain;
- and
- a signal sequence 5' to a second DNA and operatively linked to said second DNA, wherein said second DNA encodes the variable domain only of an antibody light chain, said light chain variable domain being complementary to said heavy chain variable domain;
- (b) transforming a eukaryotic host cell with said eukaryotic expression vector;
- and,
- (c) culturing the transformed host cell under conditions which cause the heavy and light chain variable domains encoded by said vector to be synthesized, correctly assembled and secreted by the host cell, such that functional Fv fragments are produced in the culture supernatant.
4 more elements
Dependencies are inferred from the claim text.