DNA encoding a truncated pneumogoccal surface protein (PspA)
AssigneeThe UAB Research Foundation
InventorsDavid E. Briles, Janet L. Yother
Life of this patent
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Abstract
A purified pneumococcal surface protein A (PspA) comprises a truncated form of the PspA protein which is immunoprotective and contains the protective epitopes of PspA. The PspA protein is soluble in physiologic solution and lacks at least the cell membrane anchor region of the whole protein. The protein is formed by insertion-duplication of mutagenesis of S. pneumoniae with pspA gene and expression of the truncated protein into the growth medium.
Claims13
An isolated DNA molecule consisting of a nuclaotide sequence coding for an immunoprotective pneumococcal surface protein A (PspA) wherein said PspA comprises the sequence of FIG. 3 (SEQ ID NO:
- 2).
An isolated DNA molecule consisting of a nucleotide sequence coding for an .alpha.-helical coiled coil region of pneumocaccal surface protein A (PspA) wherein said PspA comprises the sequence of FIG. 3 (SEQ ID NO:
- 2).
An isolated DNA molecule consisting of a nucleotide sequence coding for a proline rich region and repeat region of pneumococcal surface protein A (PspA) wherein said PspA comprises the sequence of FIG. 3 (SEQ ID NO:
- 2).
An isolated DNA molecule consisting of a nucleotide sequence coding for a truncated form of whole pneumccoccal surface protein A (PspA) wherein said whole PspA comprises the sequence of FIG. 3 (SEQ ID NO:
- 2), wherein said truncated PspA contains immunoprotective epitopes and has the cell membrane anchor region absent therefrom.
An isolated DNA molecule consisting of a nucleotide sequence coding for a truncated form of whole pneumococcal surface protein A (PspA) wherein said whole PspA comprises the sequence of FIG. 3 (SEQ ID NO:
- 2) wherein said truncated PspA contains about 50% of the whole PspA protein and has the cell membrane anchor region, the repeats region, and the proline region absent therefrom.
An isolated DNA molecule consisting of a nucleotide sequence coding for a truncated form of whole pneumococcal surface protein A (PspA) said whole PspA comprises the sequence of FIG. 3 (SEQ ID NO:
- 2), wherein said truncated PspA contains immunoprotective epitopes and about 90% of the whole PspA protein and has the cell anchor region absent therefrom.
Dependencies are inferred from the claim text.