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US 5,965,399 AGranted
Cloning and expression of rat liver and porcine liver ribonuclease inhibitor
AssigneeLife Technologies Corporation
InventorsDeb K. Chatterjee, Harini Shandilya
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Abstract
The invention relates to the cloning of a gene encoding rat liver ribonuclease inhibitor (RI), and its expression in a cellular host. In addition, the invention relates to the successful cloning of a gene encoding porcine liver RI, and its expression in a cellular host.
Claims21
An isolated nucleic acid molecule comprising a nucleotide sequence encoding a ribonuclease inhibitor (RI), wherein said nucleotide sequence is selected from the group consisting of:
- (a) the sequence set forth in SEQ ID NO:1, wherein T can also be U;
- (b) the nucleotide sequence contained in NRRL Deposit No. B-21612;
- and
- (c) a nucleic acid sequence complementary to (a) or (b).
1 more element
A method of obtaining substantially RNase free recombinant ribonuclease inhibitor, said method comprising
- (a) culturing a recombinant host comprising a nucleotide sequence encoding a ribonuclease inhibitor under conditions sufficient to express said recombinant ribonuclease inhibitor;
- (b) mixing said ribonuclease inhibitor with a binding partner molecule, with the proviso that said binding partner molecule is not an RNase;
- and
- (c) isolating said ribonuclease inhibitor from said binding partner molecule,
- wherein said nucleotide sequence encoding said ribonuclease inhibitor is selected from the group consisting of (i) the nucleotide sequence set forth in SEQ ID NO:1, wherein T can also be U;
- (ii) the nucleotide sequence contained in NRRL Deposit No. B-21612;
- and (iii) a nucleic acid sequence complementary to (a) or (b).
4 more elements
Dependencies are inferred from the claim text.