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AssigneeNational Institute of Immunology
InventorsSeyed E. Hasnain, Betapudi Venkaiah, Saman Habib
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The baculovirus homologous region (hr1) sequence has been disclosed as a transcriptional enhancer of foreign gene expression. An additional copy of the hr1 element contained within a recombinant baculovirus has been demonstrated to be capable of further enhancing expression of foreign genes placed under the transcriptional control of a promoter such as the baculovirus polyhedrin and/or Drosophila hsp70 promoter. The recombinant baculovirus is stable for up to 30 passages. This invention can be employed to generate recombinant expression vector plasmids and recombinant baculoviruses that can be used to produce increased levels of recombinant protein products of therapeutic, biomedical or basic science research interest.
A method for enhancing foreign gene expression in a baculovirus expression vector system comprising infecting an insect cell with a baculovirus viral expression vector comprising two copies of enhancer sequence hr1, at least one promoter and at least one foreign gene and culturing the cell under conditions whereby the protein encoded by the foreign gene is expressed.
A method for transiently enhancing foreign gene expression using a baculovirus expression vector system comprising infecting an insect cell with wild type baculovirus and a viral expression plasmid comprising two copies of enhancer sequence hr1, at least one promoter and at least one foreign gene and culturing the cell under conditions whereby the protein encoded by the foreign gene is expressed.
A recombinant expression plasmid comprising two enhancer sequences hr1, at least one promoter and at least one foreign gene.
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