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US 5,965,388 AGranted
Secreted proteins and polynucleotides encoding them
AssigneeGenetics Institute, Inc.
InventorsKenneth Jacobs, John M. McCoy, Edward R. LaVallie, Lisa A. Racie, David Merberg, Maurice Treacy, Cheryl Evans, Michael Bowman
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Abstract
Novel polynucleotides and the proteins encoded thereby are disclosed.
Claims14
An isolated polynucleotide selected from the group consisting of:
- (a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:10,
- (b) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:10 from nucleotide 61 to nucleotide 693;
- (c) a polynucleotide comprising the nucleotide sequence of the full-length protein coding sequence of clone O289.sub.-- 1 deposited under accession number ATCC 98101;
- (d) a polynucleotide encoding the full-length protein encoded by the cDNA insert of clone O289.sub.-- 1 deposited under accession number ATCC 98101;
- (e) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:11;
- (f) a polynucleotide encoding a protein comprising a fragment of the am no acid sequence of SEQ ID NO:11, the fragment comprising the amino acid sequence of SEQ ID NO:11 from amino acid 91 to amino acid 130;
- and
- (g) a polynucleotide that hybridizes in 6.times. SSC at 65 degrees C. to any on e of the polynucleotides specified in (a)-(f).
5 more elements
A process for producing an isolated polynucleotide, wherein the process is selected from the group consisting of:
- (a) a process comprising the steps of:
- (i) preparing one or more polynucleotide probes that hybridize in 6.times. SSC at 65 degrees C. to a nucleotide sequence selected from the group consisting of:
- (aa) SEQ ID NO:10;
- (ab) SEQ ID NO:12, and
- (ac) the nucleotide sequence of the cDNA insert of clone O289.sub.-- 1 deposited under accession number ATCC 98101;
- and
- (ii) hybridizing said probe(s) to human genomic DNA in 6.times. SSC at 65 degrees C.;
- and
- (iii) isolating the DNA polynucleotides detected with the probes);
- and
- (b) a process comprising the steps of:
- (i) preparing one or more polynucleotide primers that hybridize in 6.times. SSC at 65 degrees C. to a nucleotide sequence selected from the group consisting of:
- (ba) SEQ ID NO:10;
- (bb) SEQ ID NO:12;
- and
- (bc) the nucleotide sequence of the cDNA insert of clone O289.sub.-- 1 deposited under accession number ATCC 98101;
- and
- (ii) hybridizing said primer(s) to human genomic DNA in 6.times. SSC at 65 degrees C.;
- (iii) amplifying human DNA sequences;
- and
- (iv) isolating the polynucleotide products of step (b)(iii).
18 more elements
Dependencies are inferred from the claim text.