Methods for producing Humicola lipases in aspergillus

A method for producing an Aspergillus host cell, comprising

• (a) providing a recombinant DNA vector system capable of integration into the genome of an Aspergillus strain in one or more copies, wherein the vector system comprising (i) DNA sequences encoding functions facilitating gene expression, which comprises a promoter which shows transcriptional activity in the Aspergillus strain, transcription initiation sites;
• and transcription terminator and polyadenylation functions wherein the promoter is optionally preceded by upstream activating sequences;
• and (ii) a DNA sequence encoding a Humicola lipase, which is operatively linked to the DNA sequences encoding functions facilitating gene expression;
• (b) transforming the Aspergillus strain with the recombinant DNA cloning vector system from step (a).

A method for producing a Humicola lipase, comprising

• (a) cultivating an Aspergillus host transformed with a recombinant DNA vector system, wherein the vector system comprises (i) DNA sequences encoding functions facilitating gene expression comprising a promoter showing transcriptional activity in the Aspergillus host, transcription initiation sites, and transcription terminator and polyadenylation functions, wherein the promoter is optionally preceded by upstream activating sequences;
• and (ii) a DNA sequence encoding the Humicola lipase, wherein the DNA sequences encoding functions facilitating gene expression are operatively linked to the DNA sequence encoding the Humicola lipase;
• and
• (b) recovering the Humicola lipase;
• wherein the DNA sequence encoding the Humicola lipase is
• ATG AGG AGC TCC CTT GTG CTG TTC TTT GTC TCT GCG TGG ACG GCC TTG GCC AGT CCT ATT CGT CGA GAG GTC TCG CAG GAT CTG TTT AAC CAG TTC AAT CTC TTT GCA CAG TAT TCT GCA GCC GCA TAC TGC GGA AAA AAC AAT GAT GCC CCA GCT GGT ACA AAC ATT ACG TGC ACG GGA AAT GCC TGC CCC GAG GTA GAG AAG GCG GAT GCA ACG TTT CTC TAC TCG TTT GAA GAC TCT GGA GTG GGC GAT GTC ACC GGC TTC CTT GCT CTC GAC AAC ACG AAC AAA TTG ATC GTC CTC TCT TTC CGT GGC TCT CGT TCC ATA GAG AAC TGG ATC GGG AAT CTT AAC TTC GAC TTG AAA GAA ATA AAT GAC ATT TGC TCC GGC TGC AGG GGA CAT GAC GGC TTC ACT TCG TCC TGG AGG TCT GTA GCC GAT ACG TTA AGG CAG AAG GTG GAG GAT GCT GTG AGG GAG CAT CCC GAC TAT CGC GTG GTG TTT ACC GGA CAT AGC TTG GGT GGT GCA TTG GCA ACT GTT GCC GGA GCA GAC CTG CGT GGA AAT GGG TAT GAT ATC GAC GTG TTT TCA TAT GGC GCC CCC CGA GTC GGA AAC AGG GCT TTT GCA GAA TTC CTG ACC GTA CAG ACC GGC GGA ACA CTC TAC CGC ATT ACC CAC ACC AAT GAT ATT GTC CCT AGA CTC CCG CCG CGC GAA TTC GGT TAC AGC CAT TCT AGC CCA GAG TAC TGG ATC AAA TCT GGA ACC CTT GTC CCC GTC ACC CGA AAC GAT ATC GTG AAG ATA GAA GGC ATC GAT GCC ACC GGC GGC AAT AAC CAG CCT AAC ATT CCG GAT ATC CCT GCG CAC CTA TGG TAC TTC GGG TTA ATT GGG ACA TGT CTT TAG.

A method for producing a Humicola lipase, comprising

• (a) cultivating an Aspergillus host transformed with a recombinant DNA vector system, wherein the vector system comprises (i) DNA sequences encoding functions facilitating gene expression comprising a promoter showing transcriptional activity in the Aspergillus host, transcription initiation sites, and transcription terminator and polyadenylation functions, wherein the promoter is optionally preceded by upstream activating sequences;
• and (ii) a DNA sequence encoding the Humicola lipase, wherein the DNA sequences encoding functions facilitating gene expression are operatively linked to the DNA sequence encoding the Humicola lipase;
• and
• (b) recovering the Humicola lipase;
• wherein the DNA sequence encoding the Humicola lipase is
• GAG GTC TCG CAG GAT CTG TTT AAC CAG TTC AAT CTC TTT GCA CAG TAT TCT GCA GCC GCA TAC TGC GGA AAA AAC AAT GAT GCC CCA GCT GGT ACA AAC ATT ACG TGC ACG GGA AAT GCC TGC CCC GAG GTA GAG AAG GCG GAT GCA ACG TTT CTC TAC TCG TTT GAA GAC TCT GGA GTG GGC GAT GTC ACC GGC TTC CTT GCT CTC GAC AAC ACG AAC AAA TTG ATC GTC CTC TCT TTC CGT GGC TCT CGT TCC ATA GAG AAC TGG ATC GGG AAT CTT AAC TTC GAC TTG AAA GAA ATA AAT GAC ATT TGC TCC GGC TGC AGG GGA CAT GAC GGC TTC ACT TCG TCC TGG AGG TCT GTA GCC GAT ACG TTA AGG CAG AAG GTG GAG GAT GCT GTG AGG GAG CAT CCC GAC TAT CGC GTG GTG TTT ACC GGA CAT AGC TTG GGT GGT GCA TTG GCA ACT GTT GCC GGA GCA GAC CTG CGT GGA AAT GGG TAT GAT ATC GAC GTG TTT TCA TAT GGC GCC CCC CGA GTC GGA AAC AGG GCT TTT GCA GAA TTC CTG ACC GTA CAG ACC GGC GGA ACA CTC TAC CGC ATT ACC CAC ACC AAT GAT ATT GTC CCT AGA CTC CCG CCG CGC GAA TTC GGT TAC AGC CAT TCT AGC CCA GAG TAC TGG ATC AAA TCT GGA ACC CTT GTC CCC GTC ACC CGA AAC GAT ATC GTG AAG ATA GAA GGC ATC GAT GCC ACC GGC GGC AAT AAC CAG CCT AAC ATT CCG GAT ATC CCT GCG CAC CTA TGG TAC TTC GGG TTA ATT GGG ACA TGT CTT TAG.