Nucleic acid encoding secreted Mac-2 binding glycoprotein
AssigneeChiron Corporation
InventorsKirston E. Koths, Robert F. Halenbeck, Eric W. Taylor, Alice M. Wang, Clayton L. Casipit
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Abstract
A purified glycoprotein complex of over 1200 kD apparent native molecular weight having a sedimentation value of approximately 25S and having the ability to selectively bind human Mac-2 or interfere with PHA activation of lymphocytes, DNA sequences that encode the protein, and expression systems for expressing it, thus providing for medicaments that are useful for treating or diagnosing diseases, including cancer, infectious disease, and diseases of the immune system.
Claims22
An isolated DNA that encodes a protein comprising the amino acid sequence of residues 230-267 of SEQ ID NO:
- 10.
An isolated nucleic acid comprising at least 20 contiguous nucleotides of nucleotides 180-1934 of SEQ ID NO:9 or of the complement thereof.
A host cell comprising an exogenous nucleic acid encoding a protein comprising amino acids 230-267 of SEQ ID NO:10.
The plasmid pSKBR3-gp97 on deposit with the American Type Culture Collection with accession number 68608.
An isolated DNA encoding a protein having an amino acid sequence selected from the group consisting of:
- SEQ ID NO:10, residues 19-585 of SEQ ID NO:10, and residues 19-124 of SEQ ID NO:10.
An isolated nucleic acid selected from the group consisting of:
- a) a DNA comprising nucleotides 1-2285 of SEQ ID NO:9;
- b) a DNA comprising nucleotides 180-1934 of SEQ ID NO:9;
- c) a DNA comprising nucleotides 234-1934 of SEQ ID NO:9;
- d) a DNA having a nucleotide sequence complementary to a), b), or c);
- and
- e) an RNA comprising a nucleotide sequence corresponding to said DNA of a), b), c), or d), wherein uracil replaces thymine.
Dependencies are inferred from the claim text.