Method for measuring endogenous cytokines
AssigneeCytImmune Sciences, Inc.
InventorsLawrence Tamarkin, Giulio F. Paciotti
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Abstract
The present invention relates to methods for measuring endogenous cytokines in blood. The method accurately measures the cytokines in the blood in the presence of substances that bind the cytokines thereby causing the measurement of the cytokines by conventional methods to give inaccurate results. The present invention also includes the non-invasive measurement of cytokines in biological fluids such as saliva and nasal secretions. Finally, the present invention allows one to monitor the level of cytokines in the blood during treatment of a human or animal with cytokines.
Claims6
A method for detecting the concentration of free cytokine in a biological sample comprising the following steps in any order:
- (1) measuring the total amount of cytokine in a first portion of the sample with a competitive immunoassay comprising an antibody that binds specifically to substantially all of the cytokine in the sample, whether free or bound to a carrier molecule, wherein the antibody is immobilized on a solid phase support;
- (2) measuring the free cytokine in a second portion of the sample with a sandwich immunoassay, wherein the free cytokline is a fraction of the total cytokine.
Dependencies are inferred from the claim text.