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US 5,965,373 AGranted
Isolated dimeric fibroblast activation protein .alpha., and uses thereof
AssigneeLudwig Institute for Cancer Research
InventorsRainer Zimmermann, John E. Park, Wolfgang Rettig, Lloyd J. Old
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Abstract
The invention involves dimeric forms of the protein known as fibroblast activation protein alpha, or "FAP.alpha." and its uses.
Claims2
A method for identifying an enzyme inhibitor, comprising combining:
- (i) a dimeric molecule having fibroblast activation protein alpha (FAP.alpha.) enzymatic activity, said dimeric molecule having a molecular weight of about 170 kilodaltons as determined by SDS-PAGE;
- (ii) a substrate for said molecule;
- (iii) a substance believed to be an enzyme inhibitor, and;
- (iv) determining activity of (i) on (ii), wherein a decrease in activity when (iii) is absent indicates that said substance is an inhibitor.
1 more element
A method for identifying an enzyme inhibitor, comprising combining
- (i) a dimeric molecule having FAP.alpha. enzymatic activity, wherein said dimeric molecule consists of two protein molecules, each of which consists of the amino acid sequence of SEQ ID NO:
- 2;
- (ii) a substrate for said molecule;
- (iii) a substance believed to be an enzyme inhibitor, and;
- (iv) determining activity of (i) on (ii), wherein a decrease in activity when (iii) is absent indicates that said substance is an inhibitor.
2 more elements
Dependencies are inferred from the claim text.