Purified bradykinin B.sub.1 receptor
AssigneeMerck & Co., Inc.
InventorsDavid L. Linemeyer, John W. Hess, Joseph A. Borkowski, Kathleen K. Bierilo, John G. Menke
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Abstract
DNAs encoding bradykinin B.sub.1 receptors from mammalian cells have been cloned and characterized. The recombinant receptor is capable of forming receptors which bind des-Arg.sup.10 kallidin and other B.sub.1 -specific ligands. The DNA has been expressed in recombinant host cells which produce active recombinant protein. In addition, the recombinant host cells are utilized to establish a method for identifying modulators of the receptor activity, and receptor modulators are identified.
Claims14
A method of identifying a compound that modulates bradykinin B.sub.1 receptor activity, comprising:
- (a) combining the compound with a human bradykinin B.sub.1 receptor comprising the amino acid sequence as set forth in SEQ ID NO:2;
- and
- (b) measuring an effect of the compound on the receptor.
A method of identifying a compound that modulates bradykinin B.sub.1 receptor activity, comprising:
- (a) combining the compound with a cell expressing a recombinant bradykinin B.sub.1 receptor comprising the amino acid sequence as set forth in SEQ ID NO:2;
- and
- (b) measuring an effect of the compound on the receptor.
A human bradykinin B1 receptor protein substantially free from other human proteins which comprises the amino acid sequence a set forth in SEQ ID NO:2.
A human bradykinin B1 receptor protein substantially free from other human proteins having the amino acid sequence as set forth in SEQ ID NO:2.
Dependencies are inferred from the claim text.