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US 5,965,363 AGranted
Methods of preparing nucleic acids for mass spectrometric analysis
AssigneeGenetrace Systems, Inc.
InventorsJoseph Albert Monforte, Thomas Andrew Shaler, Yuping Tan, Christopher Hank Becker
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Abstract
This invention relates to methods for screening nucleic acids for polymorphisms by analyzing amplified target nucleic acids using mass spectrometric techniques and to procedures for improving mass resolution and mass accuracy of these methods of detecting polymorphisms.
Claims5
A mass spectrometric analysis kit for preparing one or more target nucleic acids comprising:
- (a) a first primer capable of binding a first strand of one of said target nucleic acids at a region 5' to a region of interest of said target nucleic acid;
- (b) a second primer capable of binding a second strand complementary to said first strand at a region 5' to said region of interest of said target nucleic acid;
- (c) a DNA polymerase capable of extending said primers to form primer extension products of said first and second primers;
- wherein at least one of said first or second primers comprises an exonuclease blocking moiety wherein the exonuclease blocking moiety does not include a type II restriction endonuclease recognition site.
1 more element
Dependencies are inferred from the claim text.