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US 5,965,356 AGranted
Herpes simplex virus type specific seroassay
AssigneeUniversity of Maryland, Baltimore
InventorsLaure Aurelian, Cynthia Smith
Life of this patent
Estimating the term…
Abstract
A recombinant virus (ICP10.DELTA.RR) has been developed which is useful in a Western blot assay to specifically detect antibody to HSV-2, even the presence of co-existing antibody to HSV-1.
Claims15
A method for distinguishing between HSV-1 and HSV-2 antibodies in a serum sample comprising the steps of:
- (a) contacting the serum with an antigen comprising the protein kinase domain of the ICP10 subunit of HSV-2 (SEQ. ID. NO.1) having a marker fused thereto;
- (b) using said antigen to detect the presence of HSV-2 antibody in the serum the HSV-2 antibody recognizing said antigen and binding thereto;
- and
- c) examining the antigen for the presence of said bound antibody, wherein the presence of said bound antibody indicates the presence of HSV-2 antibody in serum.
1 more element
A kit for detecting the presence of HSV-2 antibodies consisting essentially of:
- (a) an antigen comprising the protein kinase domain of the ICP10subunit of HSV-2 (SEQ. ID. NO. 1) having a marker fused thereto;
- (b) serologic assay components;
- and
- (c) written instructions for using said antigen for detecting the presence of HSV-2 antibody in a serum sample the HSV-2 antibody recognizing said antigen and binding thereto, and examining the antigen for the presence of said bound antibody, wherein the presence of said bound antibody indicates the presence of HSV-2 antibody in serum,
- wherein anti-human IgG or anti-human IgM antibodies are specifically absent from said kit.
2 more elements
Dependencies are inferred from the claim text.