Epstein-Barr virus peptides and antibodies against these peptides
AssigneeAkzo Nobel N.V.
InventorJaap Michiel Middeldorp
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Abstract
The invention relates to a peptide or fragment thereof which is immunochemically reactive with Epstein-Barr Virus (EBV) antibodies. A new monoclonal antibody directed to said peptide or fragment thereof is also part of the invention. The invention also relates to a method for the detection of EBV or anti-EBV antibodies in a test fluid and also to an immunochemical reagent comprising a peptide, a fragment or a polypeptide according to the invention and a test kit to be used when applying the said detection methods. Detection of EBV in a test fluid or tissue specimen using antibodies, monoclonal and polyclonal; directed to the said peptide, which have the characteristics of detecting both native and denatured intact, functional EBNA-1 protein is also part of said invention.
Claims15
A peptide comprising one or more of the amino acid sequences selected from the group consisting of SEQ ID NOs:
- 3, 4, 5 and 6, provided that said peptide does not contain any amino acid sequence of EBNA-1 of Epstein-Barr Virus, EBV, other than the sequences of SEQ ID NOs:
- 3, 4, 5 and 6.
A monoclonal antibody that specifically binds the same epitope on EBNA-1 as monoclonal antibodies produced by the hybridoma cell line deposited with the European Collection of Animal Cell Cultures (ECACC), Porton Down (UK), under deposit No. 92071613.
An immortalized cell line deposited with the European Collection of Animal Cell Cultures (ECACC), Porton Down (UK), under deposit No. 92071613.
Dependencies are inferred from the claim text.